Table 2: Representative examples illustrating simplex qPCR methods targeting GMO. Those validated at the EU level are indicated by an asterisk. Screening markers used in the CoSYPS are indicated by ~.

MethodsChemistriesTargetsReferences

Screening markers

Plant-specificSYBR GreenRBCL[37]
Taxon-specificSYBR GreenLEC[35]
SYBR GreenADH[37]
SYBR GreenCRU[37]
SYBR GreenPLD[37]
SYBR GreenSAD1[35]
SYBR GreenGLU[35]
Element-specificSYBR Greenp35S[31]
TaqManp35S[38]
SYBR GreentNOS[31]
TaqMantNOS[38]
SYBR GreenpFMV[39]
TaqManpFMV[35]
SYBR GreenpNOS[39]
SYBR Greent35S In-house
SYBR GreenCry1Ab/Ac[40]
TaqManCry1A(b)[35]
SYBR GreenCry3Bb[34]
SYBR Greenpat[40]
TaqManpat[35]
SYBR Greenbar[40]
TaqManbar[35]
SYBR GreenCP4-EPSPS[40]
SYBR Greent35S pCAMBIA[33]
SYBR GreennptII[35]
Construct-specificSYBR Green gat-tpinII[34]
Virus-specificSYBR GreenCRT In-house

Event-specific methods

GM-specificTaqManMaize (Zea mays)3272[35]
TaqManMaize (Zea mays)5307[35]
TaqManMaize (Zea mays)98140[35]
TaqManMaize (Zea mays)Bt11[35]
TaqManMaize (Zea mays)Bt176[35]
TaqManMaize (Zea mays)DAS-40278-9[35]
TaqManMaize (Zea mays)DAS-59122-7[35]
TaqManMaize (Zea mays)GA21[35]
TaqManMaize (Zea mays)LY038[35]
TaqManMaize (Zea mays)MIR162[35]
TaqManMaize (Zea mays)MIR604[35]
TaqManMaize (Zea mays)MON810[35]
TaqManMaize (Zea mays)MON863[35]
TaqManMaize (Zea mays)MON87460[35]
TaqManMaize (Zea mays)MON88017[35]
TaqManMaize (Zea mays)MON89034[35]
TaqManMaize (Zea mays)NK603[35]
TaqManMaize (Zea mays)T25[35]
TaqManMaize (Zea mays)TC1507[35]
TaqManSoybean (Glycine max)A2704-12[35]
TaqManSoybean (Glycine max)A5547-127[35]
TaqManSoybean (Glycine max)BPS-CV-127[35]
TaqManSoybean (Glycine max)DAS68416-4[35]
TaqManSoybean (Glycine max)DP-305423-1[35]
TaqManSoybean (Glycine max)DP-356043-5[35]
TaqManSoybean (Glycine max)FG72[35]
TaqManSoybean (Glycine max)GTS40-3-2[35]
TaqManSoybean (Glycine max)MON87701[35]
TaqManSoybean (Glycine max)MON87705[35]
TaqManSoybean (Glycine max)MON87708[35]
TaqManSoybean (Glycine max)MON87769[35]
TaqManSoybean (Glycine max)MON89788[35]
TaqManCotton (Gossypium hirsutum)281-24-236[35]
TaqManCotton (Gossypium hirsutum)3006-210-23[35]
TaqManCotton (Gossypium hirsutum)GHB119[35]
TaqManCotton (Gossypium hirsutum)GHB614[35]
TaqManCotton (Gossypium hirsutum)LLCOTTON25[35]
TaqManCotton (Gossypium hirsutum)MON531[35]
TaqManCotton (Gossypium hirsutum)MON1445[35]
TaqManCotton (Gossypium hirsutum)MON15985[35]
TaqManCotton (Gossypium hirsutum)MON88913[35]
TaqManCotton (Gossypium hirsutum)T304-40[35]
TaqManOilseed rape (Brassica napus)73496[35]
TaqManOilseed rape (Brassica napus)GT73[35]
TaqManOilseed rape (Brassica napus)MON88302[35]
TaqManOilseed rape (Brassica napus)Ms1[35]
TaqManOilseed rape (Brassica napus)Ms8[35]
TaqManOilseed rape (Brassica napus)Rf1[35]
TaqManOilseed rape (Brassica napus)Rf2[35]
TaqManOilseed rape (Brassica napus)Rf3[35]
TaqManOilseed rape (Brassica napus)T45[35]
TaqManOilseed rape (Brassica napus)Topas 19/2[35]
TaqManPotato (Solanum tuberosum)EH92-527-1[35]
TaqManRice (Oryza sativa)LLRICE62[35]
TaqManSugar beet (Beta vulgaris)H7-1[35]

ADH (alcohol dehydrogenase I gene from maize); bar (phosphinothricin-N-acetyltransferases gene from Streptomyces hygroscopicus); CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain); CRT (reverse transcriptase gene from the cauliflower mosaic virus); CRU (cruciferin gene from colza); Cry (gene encoding the Bacillus thuringiensis δ-endotoxin); gat-tpinII (junction sequence between the glyphosate N-acetyltransferase of Bacillus licheniformis and the terminator of the Solanum tuberosum proteinase inhibitor); GLU (glutamine synthetase gene from sugar beet); LEC (lectin gene from soybean); nptII (neomycin phosphotransferase II gene); p35S (promoter of the 35 S cauliflower mosaic virus); pat (phosphinothricin-N-acetyltransferases gene from Streptomyces viridochromogenes); pFMV (promoter of the figwort mosaic virus); phy (phytase gene from maize); PLD (phospholipase D gene from rice); pNOS (promoter of the nopaline synthase gene); RBCL (ribulose-1,5-biphosphate carboxylase oxygenase); SAD1 (stearoyl-acyl carrier protein desaturase gene from cotton); t35S (terminator of the cauliflower mosaic virus); t35S pCAMBIA (terminator of the cauliflower mosaic virus from pCAMBIA vector); tNOS (terminator of the nopaline synthase gene).