Review Article

Current and New Approaches in GMO Detection: Challenges and Solutions

Table 8

Representative examples illustrating simplex LAMP strategies targeting GMO.

MethodsTargetsReferences

Taxon-specificADH[76]
LEC[77, 78]
PLD[79]
IVR[80]

Element-specificp35S [76, 8186]
pFMV[83, 86]
aadA[83]
uidA[83]
nptII[83, 86]
Cry1Ab[87]
tNOS[76, 78, 82, 84, 86]
pNOS[82]
bar[84, 86]
pat[86]
Cry1Ac[86]
CP4-EPSPS[86]
Cry2A[88]
Cry3A[88]
phy[89]

Construct-specificp35S/EPSPS[82]

Event-specificMs8[82]
Rf3[82]
MON89788[77, 78, 84]
GTS 40-3-2[77, 78, 84]
DAS-59122-7[80, 84]
MON863[80, 84]
TC1507[80, 84]
T25 [80, 90]
Bt11[80]
Bt176[80]
MON810[80]
B73-6-1[91]
KMD1[79]
Kefeng-6[79]
TT51-1[79]

aadA (aminoglycoside 3′-adenylyltransferase); ACC (acetyl-CoA-carboxylase gene from colza); ADH (alcohol dehydrogenase I gene from maize); bar (phosphinothricin-N-acetyltransferases gene from Streptomyces hygroscopicus); CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain); Cry (gene encoding the Bacillus thuringiensis δ-endotoxin); IVR (invertase gene from maize); LEC (lectin gene from soybean); nptII (neomycin phosphotransferase II gene); p35S (promoter of the 35 S cauliflower mosaic virus); pat (phosphinothricin-N-acetyltransferases gene from Streptomyces viridochromogenes); pFMV (promoter of the figwort mosaic virus); phy (phytase gene from maize); PLD (phospholipase D gene from rice); pNOS (promoter of the nopaline synthase gene); tNOS (terminator of the nopaline synthase gene); uidA (β-glucuronidase).