|
DNA walking approaches | Characterized regions | Targets | References |
|
Restriction-based methods |
|
Inverse PCR | Transgene flanking regions | Bt11 | [92, 93] |
|
Cassette PCR | Transgene flanking regions | GTS40-3-2 | [94] |
Transgene flanking regions | GT73 | [95] |
Transgene flanking regions | MON1445 | [96] |
Transgene flanking regions | TC-19 | [97] |
Transgene flanking regions | TT51-1 | [98] |
Transgene flanking regions | KeFeng-6 | [99] |
Transgene flanking regions | KeFeng-8 | [100] |
Transgene flanking regions | B73-6-1 | [101] |
Transgene flanking regions | B72-8-11 | [102] |
Transgene flanking regions | B72-8-11b | [103] |
Transgene flanking regions | LY038 | [104] |
Transgene flanking regions | MON89788 | [104] |
Transgene flanking regions | 3272 | [104] |
Transgene flanking regions and unnatural element associations | CHB-351 | [105, 106] |
Transgene flanking regions and unnatural element associations | Bt176 | [95, 106] |
Transgene flanking regions and unnatural element associations | GA21 | [95, 106] |
Transgene flanking regions and unnatural element associations | Bt11 | [95, 106] |
Transgene flanking regions and unnatural element associations | T25 | [106, 107] |
Transgene flanking regions and unnatural element associations | MON810 | [106, 108] |
Transgene flanking regions and unnatural element associations | DAS-59122-7 | [104, 106] |
Unnatural element associations | MON88017 | [106] |
Unnatural element associations | MON863 × NK603 | [106] |
Unnatural element associations Unnatural element associations | MON863 × NK603 × MON810 | [106] |
Unnatural element associations | NK603 | [106] |
Unnatural element associations | MON863 | [106] |
|
Extension-based methods |
|
LT-RADE | Transgene flanking regions and unnatural element associations | MON810 | [109, 110] |
Transgene flanking regions and unnatural element associations | LLRICE62 | [109, 110] |
Transgene flanking regions and unnatural element associations | T45 | [110] |
Transgene flanking regions and unnatural element associations | A2704-12 | [110] |
Transgene flanking regions and unnatural element associations | LLCOTTON25 | [110] |
|
PCR-based methods |
|
TAIL-PCR | Transgene flanking regions | MON863 | [111, 112] |
Transgene flanking regions | KeFeng-6 | [113] |
Transgene flanking regions | B73-6-1 | [114] |
|
SiteFinding PCR | Transgene flanking regions | KMD1 | [115] |
Unnatural element associations | MIR162 | [116] |
|
APAgene GOLD Genome Walking Kit | Transgene flanking regions and unnatural element associations | Bt rice | [33, 117, 118] |
Transgene flanking regions and unnatural element associations | MON863 | [118] |
|