BioMed Research International / 2015 / Article / Tab 9

Review Article

Current and New Approaches in GMO Detection: Challenges and Solutions

Table 9

Representative examples illustrating DNA walking strategies targeting GMO.

DNA walking approachesCharacterized regionsTargetsReferences

Restriction-based methods

Inverse PCRTransgene flanking regionsBt11[92, 93]

Cassette PCRTransgene flanking regionsGTS40-3-2[94]
Transgene flanking regionsGT73 [95]
Transgene flanking regionsMON1445[96]
Transgene flanking regionsTC-19[97]
Transgene flanking regionsTT51-1[98]
Transgene flanking regionsKeFeng-6[99]
Transgene flanking regionsKeFeng-8[100]
Transgene flanking regionsB73-6-1[101]
Transgene flanking regionsB72-8-11[102]
Transgene flanking regionsB72-8-11b[103]
Transgene flanking regionsLY038[104]
Transgene flanking regionsMON89788[104]
Transgene flanking regions3272[104]
Transgene flanking regions and unnatural element associationsCHB-351[105, 106]
Transgene flanking regions and unnatural element associationsBt176[95, 106]
Transgene flanking regions and unnatural element associationsGA21[95, 106]
Transgene flanking regions and unnatural element associationsBt11[95, 106]
Transgene flanking regions and unnatural element associationsT25[106, 107]
Transgene flanking regions and unnatural element associationsMON810[106, 108]
Transgene flanking regions and unnatural element associationsDAS-59122-7[104, 106]
Unnatural element associationsMON88017[106]
Unnatural element associationsMON863 × NK603[106]
Unnatural element associations
Unnatural element associations
MON863 × NK603 ×
MON810
[106]
Unnatural element associationsNK603[106]
Unnatural element associationsMON863[106]

Extension-based methods

LT-RADETransgene flanking regions and unnatural element associationsMON810[109, 110]
Transgene flanking regions and unnatural element associationsLLRICE62[109, 110]
Transgene flanking regions and unnatural element associationsT45[110]
Transgene flanking regions and unnatural element associationsA2704-12[110]
Transgene flanking regions and unnatural element associationsLLCOTTON25[110]

PCR-based methods

TAIL-PCRTransgene flanking regionsMON863[111, 112]
Transgene flanking regionsKeFeng-6[113]
Transgene flanking regionsB73-6-1[114]

SiteFinding PCRTransgene flanking regionsKMD1[115]
Unnatural element associationsMIR162[116]

APAgene GOLD Genome Walking KitTransgene flanking regions and unnatural element associationsBt rice[33, 117, 118]
Transgene flanking regions and unnatural element associationsMON863[118]