In Vitro Metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Clinical Field Isolates, as Evaluated by Morphology, Complement Resistance, and Infectivity to Human Macrophages

Figure 3

Metacyclogenesis as evaluated by negative selection with lectin or mAb. Parasites in growth logarithmic phase were distributed into 24-well plates ( parasites/mL) in Grace’s medium, at 26°C, in triplicates. After 2, 6, or 10 days, parasites were incubated with BPL ((a), L. braziliensis) or 3A1-La mAb ((b), L. amazonensis), and nonagglutinated parasites were considered metacyclic forms. Data represent median and minimal and maximal values of percentages of nonagglutinated forms from 3 independent experiments. , 2nd versus 6th day; 2nd versus 10th days.

(a)

(b)