In Vitro Metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Clinical Field Isolates, as Evaluated by Morphology, Complement Resistance, and Infectivity to Human Macrophages

Figure 4

Flow cytometry analyses of BPL- or 3A1-La mAb-negative selected parasites. Nonfractioned parasites (total parasites: (a)–(e)) or negative-selected parasites (nonagglutinated parasites: (f)–(j)) were evaluated by flow cytometry according to FSC × SSC, at 6th day of culture. (a) and (f) M2903; (b) and (g) IMG3; (c) and (h) PPS6m; (d) and (i) PH8; (e) and (j) MAB6. are small forms (left gate; enriched in metacyclic forms); are large forms (right gate; enriched in procyclic forms).