Research Article

Quantitative iTRAQ LC-MS/MS Proteomics Reveals the Proteome Profiles of DF-1 Cells after Infection with Subgroup J Avian Leukosis Virus

Table 3

List of significant differentially expressed proteins identified by iTRAQ analysis of DF-1 cells infected with ALV-J.

Accession numberProtein nameProtein scoreFold change in expressionProtein MWProtein PI

Cluster 1: tendency for upregulation (33)
O73612Ephrin-B1 GN=EFNB1 34.981.66736.88.87
F1P187Gephyrin (fragment) GN=GPHN 0.001.56077.45.38
P12274Nonhistone chromosomal protein HMG-14B GN=HMG14 0.001.47311.29.63
E1BTX9Serine/threonine-protein phosphatase 39.241.42973.48.34
P08286Histone H1.10 476.501.31522.011.18
E1C281PHD finger protein 6 GN=PHF6 0.001.28141.08.62
Q5ZJ02DBIRD complex subunit ZNF326 GN=ZNF326 45.041.28063.55.78
Q5ZIK4Protein yippee-like GN=YPEL5 0.001.27613.87.31
Q5F3J5Proteasome activator complex subunit 3 GN=PSME3 143.051.27429.56.19
Q5F3Z5DnaJ homolog subfamily B member 6 GN=DNAJB6 0.001.26336.78.84
F1NB51Zinc finger E-box-binding homeobox 1 GN=ZEB1 41.751.258123.15.02
F1NLA7Zinc finger CCCH domain-containing protein 11A GN=ZC3H11A 43.831.25479.08.16
F1P5W3Ephrin-B1 (Fragment) GN=EFNB1 34.981.24932.78.46
F1NXG2WW domain-binding protein 4 (Fragment) GN=WBP4 0.001.23545.25.73
P08267Ferritin heavy chain GN=FTH 65.411.22621.16.21
Q6K1L7Probable RNA-binding protein EIF1AD GN=eif1ad 0.001.21021.24.79
F1NEY0Syndecan (Fragment) GN=CPQ 45.651.20819.94.70
F1NMD7Pre-mRNA-splicing factor RBM22 GN=RBM22 21.071.20546.78.54
O93481Chromobox protein (CHCB2) GN=CBX3 0.001.19019.85.12
F1NFJ0DNA replication licensing factor MCM3 GN=MCM3 46.001.18891.35.74
E1C9E9DCN1-like protein GN=DCUN1D5 0.001.18327.25.77
F1NAQ1Vascular endothelial growth factor A GN=VEGFA 0.001.17925.19.10
F1NLU6Enhancer of mRNA-decapping protein 3 GN=EDC3 49.541.17556.07.17
P16527Myristoylated alanine-rich C-kinase substrate GN=MARCKS 30.671.17427.74.44
Q5ZMC9Nuclear distribution protein nudE homolog 1 GN=NDE1 0.001.17339.55.11
Q5ZII6Protein kish-A GN=TMEM167A 27.011.1738.08.92
Q5ZIL9KIF1-binding protein homolog GN=kbp 0.001.16769.05.21
F1NFP5Arginine-tRNA ligase, cytoplasmic GN=RARS 184.951.15875.46.98
E1C4V1ATP synthase-coupling factor 6, mitochondrial GN=ATP5J 111.051.15712.59.33
Q90595Transcription factor MafF GN=MAFF 37.091.15516.69.74
R4GJF8TAR DNA-binding protein 43 GN=TARDBP131.971.15542.26.19
Q6B7Z6Polymyositis/scleroderma autoantigen 1 GN=EXOSC9 0.001.15149.35.54
E1C7X8S-adenosylmethionine synthase GN=LOC427292 14.441.15043.26.62

Cluster 2: Tendency to down-regulation (42)
R4GKA6Collagen alpha-2(VI) chain GN=COL6A2181.180.850102.45.48
E1BXS2Guanine nucleotide-binding protein G(i) subunit alpha-1 GN=GNAI1147.690.85040.45.97
Q90927Nuclear factor 1 GN=cNFI-A40.000.85054.68.31
E1BUI0tRNA pseudouridine synthase (Fragment) GN=PUSL155.040.84933.79.64
Q90617-3Isoform LAMP-2C of Lysosome-associated membrane glycoprotein 2 GN=LAMP2149.290.84946.46.43
Q90733COUP transcription factor 2 GN=NR2F20.000.84845.48.28
P12957-2Isoform Brain l-cad of Caldesmon GN=CALD10.000.84758.88.44
F1N9D8Cathepsin B GN=CTSB133.090.84737.65.86
A4GTP0Galectin66.440.84625.78.27
Q08392Glutathione S-transferase108.740.84625.38.88
F1N965Frizzled-7 GN=FZD70.000.84262.77.99
E1C3U7Lysyl oxidase homolog 2 GN=LOXL20.000.84286.96.49
F1NGX1Integrin alpha-V GN=ITGAV172.350.841114.35.58
E1BRJ4DNA-directed RNA polymerase GN=POLR3B0.000.840127.48.54
Q8AXV1Endophilin-A1 GN=SH3GL265.550.83939.95.47
F1NMF6Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 GN=PLOD1198.960.83784.36.74
F1P2F0Collagen alpha-3(VI) chain GN=COL6A3642.870.836339.46.68
R4GFM0FERM, RhoGEF and pleckstrin domain-containing protein 1 GN=FARP10.000.835119.58.15
F1N8G4Diphthamide biosynthesis protein 2 GN=DPH20.000.83452.15.54
H9L0H3Alpha-actinin-4 (Fragment) GN=ACTN4515.200.83471.66.09
Q5F4B1Phosphoglycolate phosphatase GN=PGP39.980.83333.05.73
P56673Pituitary homeobox 1 GN=PITX120.320.83334.59.11
Q9061172 kDa type IV collagenase GN=MMP2267.530.82974.95.49
F1NME2Integrin beta GN=ITGB551.040.82888.46.71
F1NBZ7Serine/threonine-protein phosphatase GN=PPP3CA0.000.82760.65.83
P51890Lumican GN=LUM44.330.82638.66.52
B3TZC1PNPLA7 GN=PNPLA70.000.821147.67.17
Q5ZLG0Acetoacetyl-CoA synthetase GN=AACS189.470.81874.36.49
F1NLD4Inhibitor of nuclear factor kappa-B kinase subunit alpha GN=CHUK0.000.81786.16.32
F1NFE0Collagen alpha-1(VI) chain GN=COL6A1110.520.812107.95.90
F1N9N4Stathmin-3 GN=NPC234.960.80816.26.51
F1NPX5SH3 domain-binding glutamic acid-rich-like protein (Fragment) GN=SH3BGRL106.540.80212.94.88
P01038Cystatin39.090.79915.37.69
Q8QG94Suppressor of fused GN=SUFU46.780.78653.75.33
Q90Y35-2Isoform 2 of Zinc finger protein 622 GN=ZNF6220.000.78342.66.65
F1NMZ3Hemoglobin subunit epsilon GN=HBE37.750.76116.68.91
Q5ZK77Biogenesis of lysosome-related organelles complex 1 subunit 5 GN=BLOC1S534.230.75922.67.06
F1P0D2Glutamine synthetase (Fragment) GN=LOC4172530.000.75844.97.02
F1NJT4Fibronectin GN=FN11373.060.752259.06.11
Q155F6Tumor necrosis factor-inducible protein 6 GN=TNFIP644.590.74730.76.02
F1NJT3Fibronectin GN=FN11373.060.727273.15.64
O93532Keratin, type II cytoskeletal cochlear95.260.69353.86.10

Fold change = infected/control. Fold change >1 indicates upregulation, and fold change <1 indicates downregulation.