Unravelling the RNA-Binding Properties of SAFB Proteins in Breast Cancer Cells

Figure 3

Loss of SAFB proteins affects expression of SHF and ITGB4. Knockdown of SAFB1 in MCF-7 cells increases mRNA and protein expression of SAFB2 and similarly knockdown of SAFB2 leads to increased expression of SAFB1. MC7-7 cells were transiently transfected with negative, SAFB1, SAFB2 or SAFB1 and SAFB2 siRNA. mRNA levels were measured by qRT-PCR. Data represents the average of three biological replicates ± SD. Statistical significance of mRNA expression was calculated using Student’s -test; = . (b) Protein levels were analysed by immunoblotting using SAFB1 and SAFB2 antibodies. (c) The effect of SAFB knockdown on SHF and ITGB4 expression by qRT-PCR using validated TaqMan probes specifically targeting SHF (c) or ITGB4 (d). Data represents the average of three biological replicates ± SD. Statistical significance of mRNA expression was calculated using Student’s -test; .

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