Hematopoietic Stem and Progenitor Cells Can Be Enriched by Implanting Biomaterial into Spatium Intermusculare

Figure 1

The migrating cells (MCs) contained phenotypic hematopoietic stem and progenitor cells (HSPCs). (a) Representative flow cytometric profiles of CD34⁺, Sca-1⁺, and CD34⁺Sca-1⁺ cells in the MCs and bone marrow (BM). MCs and BM were pregated for live cells and then were analyzed for CD34⁺, Sca-1⁺, and CD34⁺Sca-1⁺ expression. The percentages of CD34⁺, Sca-1⁺, and CD34⁺Sca-1⁺ cells in the total MCs () or BM () were expressed as mean ± SD; versus BM. (b) Immunofluorescence staining for CD34⁺ (red), Sca-1⁺ (green), and CD34⁺Sca-1⁺ (yellow) cells in the MCs and BM. Original magnifications ×100.

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