Hematopoietic Stem and Progenitor Cells Can Be Enriched by Implanting Biomaterial into Spatium Intermusculare

Figure 4

Hematopoietic stem and progenitor cells (HSPCs) in the migrating cells (MCs) originated in the bone marrow (BM). (a) Experimental scheme. 5 × 10⁶ BM cells were obtained from male mice and transplanted into female mice, and then sponges were implanted into the spatium intermusculare of female recipients at 24 hours after transplantation. The sponges were retrieved from recipients at 12 days after implantation and MCs were further analyzed. BM was obtained from female recipients as control. (b) Colony-forming unit (CFU) assay of MCs. Representative colonies were shown. BFU-E, colony-forming-unit-macrophage: CFU-M, colony-forming-unit-granulocyte, macrophage: GFU-GM, and colony-forming-unit-granulocyte, erythroid, macrophage, megakaryocyte: CFU-GEMM. (c) The percentage of individual Y-positive colonies in MCs was shown. The bars represented mean ± SD (); versus BM.

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