Prevention of Polyglycolic Acid-Induced Peritoneal Adhesions Using Alginate in a Rat Model

Table 8

(a) Immunohistochemical features on HBME-1 staining of mesothelial cells in Experiment 1. (b) Immunohistochemical features of CD68, CD86, and CD163 staining in three locations in Experiment 1.

(a)

Experimental groups	The single cell layer stained with HBME-1
PGA alone	+
Fibrin	++
SL	+
WL	++
NL	+++

HBME-1 stained the single cell layer covering the tissue surface facing the peritoneal cavity. The HBME-1 staining was scored as follows: +++: clearly stained; ++: stained; and +: unclear. The HBME-1 staining was poor on the surface over the lymph follicles in the fibrin group and over the PGA fibers in the PGA alone group.

(b)

Experimental groups	(1)			(2)			(3)
	CD68	CD86	CD163	CD68	CD86	CD163	CD68	CD86	CD163
PGA alone	±	±		±	±	×
Fibrin	+	±		+	±	×
SL	+++	+++	×	+++	+++	×
WL	++	++		++	++	×
NL	+	+		+	+	×

The macrophages were stained for CD68, CD86, and CD163 in three locations (1–3). The staining was described as follows: : mostly positive cells; : some positive cells; and ×: no positive cells. The visually recognized positive cells were scored as follows: +++: high; ++: medium; +: small; ±: few.
(1) Cells between the fibers in the PGA bundles.
(2) Cells in the space between the PGA bundles.
(3) Cells on the surface of the PGA fiber.