Impairment of Neutrophil Migration to Remote Inflammatory Site during Lung Histoplasmosis

Figure 1

Hc lung infection impairs neutrophils recruitment to the remote air pouch. (a and c) LTB₄ (0.1 μg) or (b and d) PAF (1 μg) were injected in 1 mL PBS, 2 days after Hc infection in C57Bl/6 mice (i.t., 5 × 10⁵ Hc yeast) (Hc-infected) or PBS (i.t., 100 μL) (noninfected). PBS was injected in air pouch as control (). Four hours after agonist inoculation in air pouch mice were killed and the cells in (a, b) air pouch and in (c, d) BALF were obtained and PMN in both compartments were counted as described in Materials and Methods. Data are the mean ± SEM of = 4–8 (a and b) or = 4–6 (c and d) versus PBS; noninfected versus Hc-infected mice.

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