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BioMed Research International
Volume 2015, Article ID 412327, 9 pages
http://dx.doi.org/10.1155/2015/412327
Research Article

mir-21 Overexpressing Mesenchymal Stem Cells Accelerate Fracture Healing in a Rat Closed Femur Fracture Model

1Department of Orthopaedics & Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong
2Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong
3The CUHK-ACC Space Medicine Centre, The Chinese University of Hong Kong, Shenzhen Research Institute, Shenzhen 518000, China
4Department of Orthopaedic Surgery, Baoan Hospital of Southern Medical University, The 8th People’s Hospital, Shenzhen 518000, China
5MOE Key Laboratory for Regenerative Medicine, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong

Received 4 November 2014; Revised 15 February 2015; Accepted 15 February 2015

Academic Editor: Jeroen Rouwkema

Copyright © 2015 Yuxin Sun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

For regular qPCR, beta-actin was used as internal control. Tested genes include OPN, Runx2, Osx, and ALP. For mir-21 quantification, U6 was as internal control. The universal primer was supplied in the kit.

  1. Supplementary Table