DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature

Figure 3

PCR products of UBP4^′: M-marker (bp) GeneRuler DNA Ladder Mix (Fermentas Life Sciences), lanes 1–8 UBP4^′ gene (276 bp UBP4^′ length and 26 bp for restriction enzymes sequence = 302 bp) PCR product: lanes 1, 2: PCR reaction with Biotools DNA polymerase and 23 cycles, lanes 3, 4: PCR reaction with Biotools DNA polymerase and 29 cycles, lanes 5, 6: PCR reaction with Biotools DNA polymerase and 29 cycles, and lanes 7, 8: PCR reaction with Taq DNA polymerase and 29 cycles.