Research Article

Genetic Polymorphisms of IL-17F and TRAF3IP2 Could Be Predictive Factors of the Long-Term Effect of Infliximab against Crohn’s Disease

Table 2

Information regarding the genotyping of tag SNPs in candidate genes.

GeneTag SNPMajor > minorSequence of primers (5′ to 3′) Annealing temperature (°C) Cycle numberAnalytical method (restriction enzyme)
Forward Reverse

IL17Ars4711998A > GTGTCCTCCAATTCCCTTTTGGAGACATGATGGGGGAAAGA6030PCR-RFLP (Bpu E I)
rs8193036C > TTCTTTCCCCCATCATGTCTCGTTCCAACCCTGCATGCTAC6030PCR-direct DNA sequencing
rs3819024G > AAGGCACAAACTCATCCATCCGTCAGAACCCAGCGTTTCAT6030PCR-direct DNA sequencing
rs2275913G > AGTTTCCGGAATTGTCTCCACCCCAGGAGTCATCGTTGTTT6030PCR-RFLP (Bsl I)
rs3819025A > GAGTTTCCGGAATTGTCTCCACAGCTGCCAGAAGAGTTATGC6030PCR-direct DNA sequencing

IL17Frs763780T > CCACTGGTGCTCTGATGAGGACTGCATCAATGCTCAAGGAA6035PCR-RFLP (Hsp 92 II)
rs766748G > AGTGGGAATGAGTGGAGGAGATCCACTCGCTAAGCTGGACT6030PCR-RFLP (Afa I)
rs12201582C > AACATTACGCAAAACCAACGATCATCCCCAGTAAGGGTCAG6030PCR-direct DNA sequencing
rs9382084T > GATCAACTTTCATCCCCCACACGCCATAGCAGTTTGTCAAG6035PCR-RFLP (Alu I)
rs722323C > TGGATTTCTCTGAGAGGTGCTGGGAAATCAGATGATGTCTGCAA6030PCR-direct DNA sequencing
rs1266828T > CCCCTGGATGGAAGAAATGAACATCAAAGCCTATGCCCCTA6030PCR-RFLP (Hpy CH4 V)
rs2294834A > CTTTGATTGGGGTCTTTTTGGGGGATTACAGGCAACTGACC6030PCR-direct DNA sequencing

IL17RArs2270241T > GCTGCGACTCCTGGACCACGTATTCCACACCGCAACTCC6035PCR-direct DNA sequencing
rs2241042A > CAGCTGCTTGCACAACTGCTACGCTTCTGGCATCTTTCTC6030PCR-RFLP (Hha I)
rs5748863G > ACAGGCGTGAGCCATGAAATTGGCGCAGGGATCTACTGTTT6135PCR-RFLP (Bsa A I)
rs5748864G > ACTTGGTCTGGGTCTTCGTTGGGAACCTCCACATGTTCCAC6030PCR-RFLP (Hpy CH4 IV)
rs2241044A > CTATGGGAACCAGAGCACCTCGGTCCCAGGATGAAGAAGGT6035PCR-RFLP (Tsp R I)
rs2241049A > GATGACCCTAGGCTGCTCCTTGCGGGGGTTAACTCCTTAGT6030PCR-RFLP (Hpy 188 I)
rs2229151G > ATCATCTACTCAGCCGACCACGGAGCACAGGACGATGATCT6030PCR-RFLP (Hpy 188 III)
rs2895332A > GGCGTCCTTGAGGCTCCATTACTGGCCCATTCAGCGTTTAC6030PCR-RFLP (Hpy CH4 IV)

IL17RCrs7627880G > ACAAGGTCTCTTGTGCTTGCGGATGCACTCATTCAGCAG5830PCR-RFLP (Mse I)
rs279545A > GCAGCCCTGGGAAAGTTAAGCTGTCAAGATCCCCACTCC6030PCR-RFLP (Bse Y I)

TRAF3IP2rs10872070T > CCAAGCCTAGGCCATAAGCAGTCCCACGTAGTCACCATTCA6030PCR-RFLP (Hsp 92 II)
rs6941014T > CAGCAGAGGGTGAGAGCATGTTTGCTGATGAGCCTGAGATG6030PCR-RFLP (Hae III)
rs1883136C > AAATAGCTTCCCTGCGGACTTGTGGGAGTTCCTGCAACAGT6030PCR-RFLP (Ssp I)
rs1040383C > GAGGCAACCAACTGGCAATACCTTTCTCCCAGGTTGCACAT6030PCR-RFLP (Xsp I)
rs9374263G > CTTTAACCAGGCCCACATGATTCAGGAGAGGAGCTGTTGGT6030PCR-RFLP (Hae III)
rs2075966A > GGAAATTGGCGATGGTATTGGTCACACCTCCAGACATTTGC6030PCR-RFLP (Xsp I)

SNP: single nucleotide polymorphism; 3′-UTR: 3′ untranslated region; PCR: polymerase chain reaction; RFLP: restriction fragment length polymorphism.