Inhibition of P-Selectin and PSGL-1 Using Humanized Monoclonal Antibodies Increases the Sensitivity of Multiple Myeloma Cells to Bortezomib

Figure 2

Blocking P-selectin and PSGL-1 decrease extravasation and decrease homing of MM cells to the BM in vivo. The effect of P-selectin- and PSGL-1-blocking antibodies on the number of circulating MM cells detected as calcein-AM-positive MM cells detected by flow cytometry at 50 minutes of blood aspiration after injection (a). The effect of blocking P-selectin and PSGL-1 on MM cell homing to the BM shown as the number of calcein-AM-positive cells detected in the BM, analyzed by flow cytometry, and normalized to untreated cells (b). Results are depicted as mean ± s.d. and statistical significance was analyzed by student -test ( mice per group). Values were considered significant for .

(a)

(b)