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BioMed Research International
Volume 2015 (2015), Article ID 430436, 8 pages
http://dx.doi.org/10.1155/2015/430436
Research Article

Effect of Recombinant Prophenin 2 on the Integrity and Viability of Trichomonas vaginalis

1Unidad Irapuato, Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN, P.O. Box 629, 36500 Irapuato, GTO, Mexico
2Division de Ciencias Naturales y Exactas, Departamento de Biologia, Universidad de Guanajuato, Col. Noria Alta, 36040 Guanajuato, GTO, Mexico
3Universidad Politécnica del Mar y la Sierra, Carretera a La Cruz km 15.5, Col. Arroyitos, La Cruz, 82700 Elota, SIN, Mexico

Received 31 July 2014; Accepted 26 September 2014

Academic Editor: J. Eleazar Barboza-Corona

Copyright © 2015 J. L. Hernandez-Flores et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Trichomonas vaginalis is the causal agent of trichomoniasis, which is associated with preterm child delivery, low birth weight, and an increased risk of infection by human papilloma virus and human immunodeficiency virus following exposure. Several reports have established increasing numbers of trichomoniasis cases resistant to metronidazole, the agent used for treatment, and it is therefore important to identify new therapeutic alternatives. Previously, our group reported the effect of tritrpticin, a synthetic peptide derived from porcine prophenin, on T. vaginalis; however, the hemolytic activity of this small peptide complicates its possible use as a therapeutic agent. In this study, we report that the propeptide and the processed peptide of prophenin 2 (cleaved with hydroxylamine) affected the integrity and growth of T. vaginalis and that pro-prophenin 2 displays some resistance to proteolysis by T. vaginalis proteinases at 1 h. Its effect on T. vaginalis as well as its low hemolytic activity and short-time stability to parasite proteinases makes prophenin 2 an interesting candidate for synergistic or alternative treatment against T. vaginalis.