The Genetic Deletion of 6q21 and PRDM1 and Clinical Implications in Extranodal NK/T Cell Lymphoma, Nasal Type

Figure 1

Identification of 6q21 and PRDM1 deletion by FISH and PRDM1 expression by immunostaining in EN-NK/T-NT cases. Dual-color FISH was performed with 6q21 probe or PRDM1 specific probe (labelled with 5-carboxyl-xrhodamine, red) and chromosome 6 centromere specific probe (labelled with 5-fluorescein, green), respectively. Case , (a), (b), and (c): one representative case showed the heterozygous codeletion of 6q21 (a, two green and one red) and PRDM1 gene (b, two green and one red) and PRDM1 negative immunostaining (c). Case , (d), (e), and (f): another representative case with the heterozygous codeletion of 6q21 (d, two green and one red) and PRDM1 gene (e, two green and one red) and PRDM1 negative immunostaining (f). (a, b, d, and e at 1000x magnification; c and f at 400x magnification).