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BioMed Research International
Volume 2015, Article ID 436319, 10 pages
http://dx.doi.org/10.1155/2015/436319
Research Article

Experimental Protoporphyria: Effect of Bile Acids on Liver Damage Induced by Griseofulvin

1Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Intendente Güiraldes 2160, 1428 Buenos Aires, Argentina
2Centro de Investigaciones sobre Porfirinas y Porfirias, CONICET-UBA, Avenida Córdoba 2351, 1120 Buenos Aires, Argentina
3Departamento de Patología, Instituto de Estudios Oncológicos, Academia Nacional de Medicina, Pacheco de Melo 3081, 1425 Buenos Aires, Argentina

Received 8 October 2014; Revised 6 January 2015; Accepted 13 January 2015

Academic Editor: Stelvio M. Bandiera

Copyright © 2015 María del Carmen Martinez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effect of bile acids administration to an experimental mice model of Protoporphyria produced by griseofulvin (Gris) was investigated. The aim was to assess whether porphyrin excretion could be accelerated by bile acids treatment in an attempt to diminish liver damage induced by Gris. Liver damage markers, heme metabolism, and oxidative stress parameters were analyzed in mice treated with Gris and deoxycholic (DXA), dehydrocholic (DHA), chenodeoxycholic, or ursodeoxycholic (URSO). The administration of Gris alone increased the activities of glutathione reductase (GRed), superoxide dismutase (SOD), alkaline phosphatase (AP), gamma glutamyl transpeptidase (GGT), and glutathione-S-transferase (GST), as well as total porphyrins, glutathione (GSH), and cytochrome P450 (CYP) levels in liver. Among the bile acids studied, DXA and DHA increased PROTO IX excretion, DXA also abolished the action of Gris, reducing lipid peroxidation and hepatic GSH and CYP levels, and the activities of GGT, AP, SOD, and GST returned to control values. However, porphyrin accumulation was not prevented by URSO; instead this bile acid reduced ALA-S and the antioxidant defense enzymes system activities. In conclusion, we postulate that DXA acid would be more effective to prevent liver damage induced by Gris.