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BioMed Research International
Volume 2015, Article ID 451981, 10 pages
http://dx.doi.org/10.1155/2015/451981
Research Article

Establishment of the MethyLight Assay for Assessing Aging, Cigarette Smoking, and Alcohol Consumption

1Department of Genomic Medicine, National Cerebral and Cardiovascular Center, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan
2Department of Cardiovascular Medicine, National Cerebral and Cardiovascular Center, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan
3Clinical Laboratory, National Cerebral and Cardiovascular Center, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan

Received 21 May 2015; Revised 13 August 2015; Accepted 5 October 2015

Academic Editor: Xia Li

Copyright © 2015 Kosuke Endo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. Bird, “DNA methylation patterns and epigenetic memory,” Genes & Development, vol. 16, no. 1, pp. 6–21, 2002. View at Publisher · View at Google Scholar · View at Scopus
  2. K. Shiota, “DNA methylation profiles of CpG islands for cellular differentiation and development in mammals,” Cytogenetic and Genome Research, vol. 105, no. 2–4, pp. 325–334, 2004. View at Publisher · View at Google Scholar · View at Scopus
  3. J. C. Mathers, G. Strathdee, and C. L. Relton, “Induction of epigenetic alterations by dietary and other environmental factors,” in Advances in Genetics, vol. 71, chapter 1, pp. 3–39, Elsevier, 2010. View at Publisher · View at Google Scholar
  4. S. Bocklandt, W. Lin, M. E. Sehl et al., “Epigenetic predictor of age,” PLoS ONE, vol. 6, no. 6, Article ID e14821, 2011. View at Publisher · View at Google Scholar · View at Scopus
  5. L. Tserel, M. Limbach, M. Saare et al., “CpG sites associated with NRP1, NRXN2 and miR-29b-2 are hypomethylated in monocytes during ageing,” Immunity and Ageing, vol. 11, article 1, 2014. View at Publisher · View at Google Scholar · View at Scopus
  6. K. W. K. Lee and Z. Pausova, “Cigarette smoking and DNA methylation,” Frontiers in Genetics, vol. 4, article 132, 2013. View at Publisher · View at Google Scholar · View at Scopus
  7. N. S. Shenker, P. M. Ueland, S. Polidoro et al., “DNA methylation as a long-term biomarker of exposure to tobacco smoke,” Epidemiology, vol. 24, no. 5, pp. 712–716, 2013. View at Publisher · View at Google Scholar · View at Scopus
  8. L. G. Tsaprouni, T.-P. Yang, J. Bell et al., “Cigarette smoking reduces DNA methylation levels at multiple genomic loci but the effect is partially reversible upon cessation,” Epigenetics, vol. 9, no. 10, pp. 1382–1396, 2014. View at Publisher · View at Google Scholar · View at Scopus
  9. R. A. Philibert, B. Penaluna, T. White et al., “A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs,” Epigenetics, vol. 9, no. 9, pp. 1212–1219, 2014. View at Publisher · View at Google Scholar · View at Scopus
  10. H. Zhang, F. Wang, H. R. Kranzler et al., “Identification of methylation quantitative trait loci (mQTLs) influencing promoter DNA methylation of alcohol dependence risk genes,” Human Genetics, vol. 133, no. 9, pp. 1093–1104, 2014. View at Publisher · View at Google Scholar · View at Scopus
  11. S. Horvath, “DNA methylation age of human tissues and cell types,” Genome Biology, vol. 14, no. 10, article R115, 2013. View at Publisher · View at Google Scholar · View at Scopus
  12. T. M. Rickabaugh, R. M. Baxter, M. Sehl et al., “Acceleration of age-associated methylation patterns in HIV-1-infected adults,” PLoS ONE, vol. 10, no. 3, Article ID e0119201, 2015. View at Publisher · View at Google Scholar
  13. C. I. Weidner, Q. Lin, C. M. Koch et al., “Aging of blood can be tracked by DNA methylation changes at just three CpG sites,” Genome Biology, vol. 15, no. 2, article R24, 2014. View at Publisher · View at Google Scholar · View at Scopus
  14. E. Olkhov-Mitsel, D. Zdravic, K. Kron, T. van der Kwast, N. Fleshner, and B. Bapat, “Novel multiplex MethyLight protocol for detection of DNA methylation in patient tissues and bodily fluids,” Scientific Reports, vol. 4, article 4432, 2014. View at Publisher · View at Google Scholar · View at Scopus
  15. K. Endo, N. Kito, Y. Fukushima, H. Weng, and N. Iwai, “A novel biomarker for acute kidney injury using TaqMan-based unmethylated DNA-specific polymerase chain reaction,” Biomedical Research, vol. 35, no. 3, pp. 207–213, 2014. View at Publisher · View at Google Scholar · View at Scopus
  16. L. L. Lam, E. Emberly, H. B. Fraser et al., “Factors underlying variable DNA methylation in a human community cohort,” Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 2, pp. 17253–17260, 2012. View at Publisher · View at Google Scholar · View at Scopus
  17. C. A. Eads, K. D. Danenberg, K. Kawakami et al., “MethyLight: a high-throughput assay to measure DNA methylation,” Nucleic Acids Research, vol. 28, no. 8, article E32, 2000. View at Publisher · View at Google Scholar · View at Scopus
  18. K. Hashimoto, S. Kokubun, E. Itoi, and H. I. Roach, “Improved quantification of DNA methylation using methylation-sensitive restriction enzymes and real-time PCR,” Epigenetics, vol. 2, no. 2, pp. 86–91, 2007. View at Publisher · View at Google Scholar · View at Scopus
  19. N. Redshaw, J. F. Huggett, M. S. Taylor, C. A. Foy, and A. S. Devonshire, “Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis,” BMC Genomics, vol. 15, article 1174, 2014. View at Publisher · View at Google Scholar
  20. R. E. Marioni, S. Shah, A. F. McRae et al., “DNA methylation age of blood predicts all-cause mortality in later life,” Genome Biology, vol. 30, pp. 16–25, 2015. View at Google Scholar
  21. S. Horvath, P. Garagnani, M. G. Bacalini et al., “Accelerated epigenetic aging in Down syndrome,” Aging Cell, vol. 14, pp. 491–495, 2015. View at Publisher · View at Google Scholar · View at Scopus
  22. M. Sugimoto, H. Tahara, T. Ide, and Y. Furuichi, “Steps involved in immortalization and tumorigenesis in human B-lymphoblastoid cell lines transformed by Epstein-Barr virus,” Cancer Research, vol. 64, no. 10, pp. 3361–3364, 2004. View at Publisher · View at Google Scholar · View at Scopus
  23. L. Tserel, R. Kolde, M. Limbach et al., “Age-related profiling of DNA methylation in CD8+ T cells reveals changes in immune response and transcriptional regulator genes,” Scientific Reports, vol. 5, article 13107, 2015. View at Publisher · View at Google Scholar
  24. R. A. Philibert, N. Terry, C. Erwin, W. J. Philibert, S. R. Beach, and G. H. Brody, “Methylation array data can simultaneously identify individuals and convey protected health information: an unrecognized ethical concern,” Clinical Epigenetics, vol. 6, article 28, 2014. View at Publisher · View at Google Scholar
  25. J. Mimura, M. Ema, K. Sogawa, and Y. Fujii-Kuriyama, “Identification of a novel mechanism of regulation of Ah (dioxin) receptor function,” Genes and Development, vol. 13, no. 1, pp. 20–25, 1999. View at Publisher · View at Google Scholar · View at Scopus
  26. R. A. Philibert, S. R. H. Beach, and G. H. Brody, “Demethylation of the aryl hydrocarbon receptor repressor as a biomarker for nascent smokers,” Epigenetics, vol. 7, no. 11, pp. 1331–1338, 2012. View at Publisher · View at Google Scholar · View at Scopus
  27. S. Zeilinger, B. Kühnel, N. Klopp et al., “Tobacco smoking leads to extensive genome-wide changes in DNA methylation,” PLoS ONE, vol. 8, no. 5, Article ID e63812, 2013. View at Publisher · View at Google Scholar · View at Scopus
  28. N. L. Benowitz, “Clinical pharmacology of nicotine: implications for understanding, preventing, and treating tobacco addiction,” Clinical Pharmacology and Therapeutics, vol. 83, no. 4, pp. 531–541, 2008. View at Publisher · View at Google Scholar · View at Scopus
  29. K. Mattsson, K. Källén, A. Rignell-Hydbom et al., “Cotinine validation of self-reported smoking during pregnancy in the Swedish Medical Birth Register,” Nicotine & Tobacco Research, 2015. View at Publisher · View at Google Scholar
  30. P. Cabarcos, I. Álvarez, M. J. Tabernero, and A. M. Bermejo, “Determination of direct alcohol markers: a review,” Analytical and Bioanalytical Chemistry, vol. 407, no. 17, pp. 4907–4925, 2015. View at Publisher · View at Google Scholar
  31. S. Kechagias, D. N. Dernroth, A. Blomgren et al., “Phosphatidylethanol compared with other blood tests as a biomarker of moderate alcohol consumption in healthy volunteers: a prospective randomized study,” Alcohol and Alcoholism, vol. 50, no. 4, pp. 399–406, 2015. View at Publisher · View at Google Scholar