Characterization of a Thioredoxin-1 Gene from Taenia solium and Its Encoding Product

Figure 3

(a) Alignment of Trx-1 from Taenia solium (Ts. GenBank accession KM401605) with other thioredoxins from Echinococcus granulosus (Eg. GenBank: AF034637.1), Schistosoma mansoni (Sm. GenBank: AAL79841.1), Sus scrofa (Ss. GenBank NM_214313.2), and Homo sapiens (Hs. GenBank AF085844.1). Identical residues are highlighted in white on a black background. The symbols in the residues indicate (-) absence and (:) homology. Tyrosine 49 where nitration occurs (■) in mammalian. Cysteines: (▲) from active site, (○) conserved cysteine in mammalian and helminths, and (⧫) only present in mammalians where S-nitrosylation occurs; likewise (⧫) it is involved in glutathionylation. In a box is the active site and underlined are the residues used for primer design to produce the Trx-1 probe. Secondary structure elements are shown above the alignment. (b) Structure model of TsTrx-1. It shows the Trx fold formed by a central domain with five-stranded -sheet, surrounded by four -helices. The model was drawn with the Swiss Model program (http://swissmodel.expasy.org/).

(a)

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