Identification and Validation of Potential New Biomarkers for Prostate Cancer Diagnosis and Prognosis Using 2D-DIGE and MS

Figure 2

2D-DIGE analysis of 14 tumor free prostate tissue samples, 12 prostatectomy samples from prostate cancer patients without relapse in a 5-year followup, and 11 prostatectomy samples from patients with relapse. (a) Overlay of 2D-DIGE gels of prostatectomy samples from tumor-free tissue areas ((b), green) and patients with prostate cancer ((c), red). (d) Overlay of 2D-DIGE gels from prostatectomy samples from patients without ((e), red) and with ((f), green) relapse. Downregulated spots in prostate cancer and prostate cancer with relapse, respectively, are annotated with “down.” Upregulated spots in these samples are annotated with “up.” Principal component analysis (PCA) of prostate cancer (red) and tumor-free tissue (green) (g) and prostate cancer samples without (red) and with relapse (green) (h).