Identification and Validation of Potential New Biomarkers for Prostate Cancer Diagnosis and Prognosis Using 2D-DIGE and MS

Figure 6

Representative immunohistochemistry of vinculin in an independent tissue microarray (TMA) obtained from the University Hospital Bonn. 116 tumor-free tissue samples, 54 prostatic intraepithelial neoplasia (PIN) lesions, 54 prostatic samples from patients without relapse (− rec), and 16 prostatectomy samples from prostate cancer patients with relapse (+ rec) were analyzed. Boxplots of the immunohistological scores of the stained tissue. (b) Percentage of each score in each analyzed patients group. For more detailed information, an adapted Remmele score was used to classify the vinculin expression. (c)–(j) Immunohistochemically stained tissue: (c) and (d) tumor-free prostatectomy samples, (e) and (f) prostatectomy samples of PIN, (g) and (h) prostatectomy samples of prostate cancer patients without relapse, and (i) and (j) prostatectomy samples of prostate cancer patients with relapse. Vinculin is significantly upregulated in peri-intraepithelial neoplasia (PIN) and prostate cancer compared to tumor-free tissue samples ().