Activation of Endocannabinoid System Is Associated with Persistent Inflammation in Human Aortic Aneurysm

Figure 3

mRNA expression of cytokines and chemokines in aortic wall. The mRNA expression of proinflammatory cytokines (a) TNFα, (b) IFNγ, and (c) IL-1β was comparable between ascending aortas from aneurysms (AAA) and patients with coronary artery disease (CAD). The mRNA expression of (d) IL-6 was lower, while (e) macrophage colony-stimulating factor (M-CSF) and (f) peroxisome proliferator-activated receptor (PPAR)γ were significantly higher in AAA than in CAD aortic tissue. The expression of (g) the anti-inflammatory cytokine IL-10 was comparable between the groups. (h) A significantly lower expression of potent mononuclear chemoattractants CCL2 and (i) nonsignificantly lower level of related chemokine CCL4 were found in AAA samples. in the AAA group and in the CAD RT-qPCR group. mRNA expression in RT-qPCR is related to controls and GAPDH using comparative ΔΔCt method. Bracket indicates .

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