Research Article

The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells

Figure 1

Viromer RED shows comparable transfection to Lipofectamine 3000 in a diversity of cell lines: HEK293, DI TNC1, and BV2 cells were transfected with pMaxGFP using Viromer RED (VIRO) or Lipofectamine 3000 with enhancer (Lipo). Transfections were performed either in serum (+FBS) or without serum (−FBS). Serum containing media was added to the cells 4–6 hr after transfection. Phase contrast and fluorescence images show a robust transfection efficiency by both reagents in HEK293 (a) that decreases in the presence of serum (a, right). The transfection efficiency was lower in the DI TNC1 cell line compared to HEK293 (b). Viromer RED transfected a higher percentage of BV2 cells with pMaxGFP vector (c) as compared to Lipofectamine 3000 with enhancer. Numbers represent percentage mean ± SD transfected cells.
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