The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells

Figure 2

Viromer RED shows comparable transfection to Lipofectamine 3000 in primary glia. Primary rat astrocytes and Schwann cells were transfected with pMaxGFP using Viromer RED (VIRO) or Lipofectamine 3000 with enhancer (Lipo). Transfections were performed in serum (+FBS). Phase contrast and fluorescence images show a comparable transfection efficiency by both reagents in primary rat astrocytes (a) and primary rat Schwann cells (b). No gross change in the morphology of astrocytes or Schwann cells was observed after transfection using the reagents. Purity of the cultures was assessed using GFAP (for astrocytes, a) and S100 (for Schwann cells, b). Numbers represent percentage mean ± SD transfected cells.

(a)

(b)