Molecular Epidemiology of Human Norovirus in Korea in 2013

Table 1

Primers used in this study for the RT-PCR and sequencing of norovirus.

Targeted gene		Reaction type	Primer name	Primer orientation	Primer sequence (5′ to 3′)	Annealing position	PCR product size
ORF1; (polymerase, RdRp)	1	One-step RT-PCR	JV12	F	ATA CCA CTA TGA TGC AGA TTA	nt 4552	327 bp
			JV13	R	TCA TCA TCA CCA TAG AAA GAG	nt 4878
	2	1st PCR	NV32	F	ATG AAT ATG AAT GAA GAT GG	nt 4226	501 bp
			NV36	R	ATT GGT CCT TCT GTT TTG TC	nt 4726
		Nested PCR	NV33	F	TAC CAC TAT GAT GCA GAT TA	nt 4280	357 bp
			NV35	R	GTT GAC ACA ATC TCA TCA TC	nt 4636
ORF2; (capsid, VP1)	1 (GI)	1st PCR	GI-F1M	F	CTG CCC GAA TTY GTA AAT GAT GAT	nt 5342	330 bp
			GI-R1M	R	CCA ACC CAR CCA TTR TAC ATY TG	nt 5671
		Nested PCR	GI-F2	F	ATG ATG ATG GCG TCT AAG GAC GC	nt 5357	315 bp
			GI-R1M	R	CCA ACC CAR CCA TTR TAC ATY TG	nt 5671
	2 (GII)	1st PCR	GII-F1M	F	GGG AGG GCG ATC GCA ATC	nt 5058	344 bp
			GII-R1M	R	CCR CCT GCA TRI CCR TTR TAC AT	nt 5401
		Nested PCR	GII-F3	F	TTG CCR GCA TRI CCR TTR TAC AT	nt 5088	314 bp
			GII-R1M	R	CCR CCT GCA TRI CCR TTR TAC AT	nt 5401
ORF1-ORF2 junction		One-step RT-PCR	NV33	F	TAC CAC TAT GAT GCA GAT TA	nt 4280	1110 bp
			G2SKR	R	CCR CCN GCA TRH CCR TTR TAC AT	nt 5389
ORF2 (capsid, VP1)		One-step RT-PCR	GII-F1M	F	GGG AGG GCG ATC GCA ATC	nt 5049	1681 bp
			GV132	R	CCR GCR AAG AAA GCR CCA GCC AT	nt 6729

F, forward; R, reverse.