Research Article
Molecular Epidemiology of Human Norovirus in Korea in 2013
Table 1
Primers used in this study for the RT-PCR and sequencing of norovirus.
| Targeted gene | | Reaction type | Primer name | Primer orientation | Primer sequence (5′ to 3′) | Annealing position | PCR product size |
| ORF1 (polymerase, RdRp) | 1 | One-step RT-PCR | JV12 | F | ATA CCA CTA TGA TGC AGA TTA | nt 4552 | 327 bp | JV13 | R | TCA TCA TCA CCA TAG AAA GAG | nt 4878 | 2 | 1st PCR | NV32 | F | ATG AAT ATG AAT GAA GAT GG | nt 4226 | 501 bp | NV36 | R | ATT GGT CCT TCT GTT TTG TC | nt 4726 | Nested PCR | NV33 | F | TAC CAC TAT GAT GCA GAT TA | nt 4280 | 357 bp | NV35 | R | GTT GAC ACA ATC TCA TCA TC | nt 4636 |
| ORF2 (capsid, VP1) | 1 (GI) | 1st PCR | GI-F1M | F | CTG CCC GAA TTY GTA AAT GAT GAT | nt 5342 | 330 bp | GI-R1M | R | CCA ACC CAR CCA TTR TAC ATY TG | nt 5671 | Nested PCR | GI-F2 | F | ATG ATG ATG GCG TCT AAG GAC GC | nt 5357 | 315 bp | GI-R1M | R | CCA ACC CAR CCA TTR TAC ATY TG | nt 5671 | 2 (GII) | 1st PCR | GII-F1M | F | GGG AGG GCG ATC GCA ATC | nt 5058 | 344 bp | GII-R1M | R | CCR CCT GCA TRI CCR TTR TAC AT | nt 5401 | Nested PCR | GII-F3 | F | TTG CCR GCA TRI CCR TTR TAC AT | nt 5088 | 314 bp | GII-R1M | R | CCR CCT GCA TRI CCR TTR TAC AT | nt 5401 |
| ORF1-ORF2 junction | | One-step RT-PCR | NV33 | F | TAC CAC TAT GAT GCA GAT TA | nt 4280 | 1110 bp | G2SKR | R | CCR CCN GCA TRH CCR TTR TAC AT | nt 5389 |
| ORF2 (capsid, VP1) | | One-step RT-PCR | GII-F1M | F | GGG AGG GCG ATC GCA ATC | nt 5049 | 1681 bp | GV132 | R | CCR GCR AAG AAA GCR CCA GCC AT | nt 6729 |
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F, forward; R, reverse.
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