Full GMP-Compliant Validation of Bone Marrow-Derived Human CD133+ Cells as Advanced Therapy Medicinal Product for Refractory Ischemic Cardiomyopathy
Table 2
Recovery of CD133+ cells.
Batch #
Starting material
Pre-CliniMACS selection
Post-CliniMACS selection
Absolute number of CD133+ cells (×106)
Cell recovery 1 (%)
Cell recovery 2 (%)#
Cell recovery 3 (%)
PTC-CD133-052
180.69
50.29
18.37
9.24
PTC-CD133-055
123.68
38.25
20.10
7.69
PTC-CD133-068
38.13
85.21
30.05
25.61
PTC-CD133-085
44.89
78.99
20.69
16.34
PTC-CD133-090
15.40
70.22
35.45
24.89
PTC-CD133-098
65.81
51.23
30.05
15.40
PTC-CD133-109
97.54
68.17
51.20
34.90
PTC-CD133-116
40.61
61.62
56.77
34.98
Mean
75.84
63.00
32.83
21.13
Standard deviation
54.94
15.78
14.40
10.65
Median
55.35
64.89
30.05
20.62
Minimum value
15.40
38.25
18.37
7.69
Maximum value
180.69
85.21
56.77
34.98
Preselection is referred to cells obtained after Ficoll centrifugation. Cell recovery from starting material. #Cell recovery from pre-CliniMacs selection. Cell recovery from starting material.
