A Tissue Retrieval and Postharvest Processing Regimen for Rodent Reproductive Tissues Compatible with Long-Term Storage on the International Space Station and Postflight Biospecimen Sharing Program

<div>Morphological analysis of mouse testis after different postfixative manipulations. Sections of adult mouse testis were stained with HE (all at 60x; magnification bar is 40 <i>μ</i>m). (a) Control; (b) slow rehydration-dehydration stepwise replacement of ETOH-PBS-ETOH; (c) slow Re-ETOH<i> only</i> stepwise replacement; (d) rapid ETOH-PBS-ETOH transition. Sertoli cell (Se), spermatogonia (Sp), spermatocytes (Sc), spermatids (Sd), and Leydig cell (L).<i> Black arrows</i>—abnormal open spaces in seminiferous epithelium;<i> yellow arrows</i>—abnormal wavy and thinner basement membrane.</div>

BioMed Research International

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Figure 4

Figure 4: A Tissue Retrieval and Postharvest Processing Regimen for Rodent Reproductive Tissues Compatible with Long-Term Storage on the International Space Station and Postflight Biospecimen Sharing Program 

Figure 4 | A Tissue Retrieval and Postharvest Processing Regimen for Rodent Reproductive Tissues Compatible with Long-Term Storage on the International Space Station and Postflight Biospecimen Sharing Program 