A Tissue Retrieval and Postharvest Processing Regimen for Rodent Reproductive Tissues Compatible with Long-Term Storage on the International Space Station and Postflight Biospecimen Sharing Program

Table 1

Percent progressive motility (±SD) of mouse cauda epididymal sperm after exposure of epididymis to various conditions.

Time after euthanasia	In carcass	On ice	At room temperature
0 h	23 ± 18	25 ± 26	26 ± 19
0.5 h	28 ± 13	22 ± 13	27 ± 12
1.0 h	31 ± 3	32 ± 19	31 ± 9
1.5 h	27 ± 6	47 ± 9	30 ± 11
2.0 h	26 ± 7	18 ± 9	28 ± 14
2.5 h	26 ± 9	23 ± 12	25 ± 16

Tissue was submerged in Ham’s F-10 medium in a 15 mL tissue culture tube which was placed on ice or at room temperature. Values are mean ± standard deviation ( mice at each time point). There was no significant difference in motility between the three testing conditions at each time point.