Research Article
Melanoma Cells Homing to the Brain: An In Vitro Model
Figure 3
The panel shows the localization of two markers representative of the blood brain barrier tight junctions. (a) Expression of zonula occludens-1 (ZO-1) focalized in membrane producing a linear staining at cell boundaries and (b) merge of ZO-1 and nuclear staining (10x magnification); (B) magnification detail of ZO-1 and DAPI staining (40x magnification); (c) membrane-cytoskeletal protein of focal adhesion plaques vinculin protein; and (d) merge of vinculin and nuclear staining (10x magnification); (D) magnification detail of vinculin and DAPI staining (40x magnification). DAPI: blue; ZO-1: green; vinculin: green. Scale bar: 100 μm.
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