Melanoma Cells Homing to the Brain: An In Vitro Model

Table 1

Differentially expressed genes in M1 and M2 cells as obtained by the metastasis specific real time PCR-based array (see materials and methods, Section 2). Data are expressed as fold decrease (a) or increase (b) in M2 compared to M1.

Symbol	Gene name	Fold	Function
(a)
SYK	Spleen tyrosine kinase	−91.72	AM
MCAM	Melanoma cell adhesion molecule	−29.76	AM
VEGFA	Vascular endothelial growth factor A	−7.85	AM
CTNNA1	Catenin (cadherin-associated protein), alpha 1	−6.51	AM
ITGB3	Integrin, beta 3	−6.46	AM
FAT1	FAT atypical cadherin 1	−5.36	AM
HPSE	Heparanase	−5.05	EMD
CD44	CD44 molecule	−5.05	AM
TIMP2	Metallopeptidase inhibitor 2	−4.72	EMD
COL4A2	Collagen, type IV, alpha 2	−4.60	EMD
APC	Adenomatous polyposis coli	−4.02	AM
PNN pinin	Desmosome associated protein	−3.53	AM
MMP3	Matrix metallopeptidase 3 (stromelysin 1, progelatinase)	−2.58	EMD
MTSS1	Metastasis suppressor 1	−2.57	AM
(b)
CDH1	Cadherin 1, type 1, E-cadherin	533	AM
MMP2	Matrix metallopeptidase 2 (gelatinase A, 72 kDa gelatinase, 72 kDa type IV collagenase)	54.35	EMD
ITGA7	Integrin, alpha 7	15.54	AM
CDH6	Cadherin 6, type 2, K-cadherin (fetal kidney)	8.23	AM
MMP10	Matrix metallopeptidase 10 (stromelysin 2)	6.83	EMD
TIMP3	TIMP metallopeptidase inhibitor 3	4.92	EMD
CDH11	Cadherin 11, type 2, OB-cadherin (osteoblast)	3.97	AM
MMP9	matrix metallopeptidase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase)	3.58	EMD
MMP11	Matrix metallopeptidase 11 (stromelysin 3)	3.55	EMD
MMP13	Matrix metallopeptidase 13 (collagenase 3)	3.31	EMD
MMP7	Matrix metallopeptidase 7 (matrilysin, uterine)	2.61	EMD
MGAT5	Mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetylglucosaminyltransferase	2.22	AM
FN1	Fibronectin 1	2.08	AM
CTSL1	Cathepsin L	2.06	EMD