Table 3: Four mutations with significant differences in mutant allele frequency between the three panels.

Cell lineGeneTranscriptVariantFluidigm custom
panel
SureSeq Solid Tumour panel Ion AmpliSeq Cancer Hotspot PanelSanger sequencing zygosity status

97-7FGFR3 NM_00142.4c.746C>GN/A81.4%34.6%Heterozygous
KU-19-19NRAS NM_002524.3c.182A>G48.2%50.5%86.3%Heterozygous
J82TP53 NM_000546.5c.960G>C85.7%51.3%N/AHeterozygous
LUCC3TP53 NM_000546.5c.840_841insAA57.7%58.9%96.4%Heterozygous