Research Article

Safety Evaluation of the Coagulase-Negative Staphylococci Microbiota of Salami: Superantigenic Toxin Production and Antimicrobial Resistance

Figure 2

Uniplex, duplex, and multiplex PCR screening for the detection of enterotoxin genes in CNS strains from salami. (a) Lane M, 100 bp DNA ladder plus (Fermentas, Foster City, CA, USA); lane 1, S. aureus ATCC 29231 harboring sea gene; lane 2, S. aureus NCTC 10654 harboring seb gene; lane 3, S. aureus ATCC19095 harboring the sec gene; lane 4, S. aureus ATCC 13563 harboring the sed gene; and lane 5, S. aureus ATCC 27664 harboring the see gene. (b) Lane M 100 bp DNA ladder plus; lane 1, Staphylococcus spp.; lane 2, S. carnosus NR116434; lane 3, S. carnosus KJ862002; lane 4, S. carnosus KJ862002; and lane 5, S. carnosus KJ862002.1. (c) Lane M, 100 bp DNA ladder plus; lane 1, S. aureus ATCC 19095 harboring seg, seh, and sei genes. (d) Lane M, 100 bp DNA ladder plus; lane 1, S. saprophyticus AB697717.1; lane 2, S. epidermidis KF 600589.1; and lane 3, S. sciuri JX966436.1. (e) Lane M, 100 bp DNA ladder plus; lane 1, S. xylosus KF198080.1; lane 2, S. saprophyticus KJ699151.1. (f) Lane M, 100 bp DNA ladder plus; lane 1, S. aureus ATCC 27154 harboring selj, slem, seln, and selo genes. (g) Lane M, 100 bp DNA ladder plus; lane 1, S. xylosus KF198080.1; and lane 2, S. saprophyticus subsp. bovis KJ699151.1.
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