Safety Evaluation of the Coagulase-Negative Staphylococci Microbiota of Salami: Superantigenic Toxin Production and Antimicrobial Resistance

Table 1

Primer set for V3 16S rDNA sequencing and PCR/real time RT-PCR tests targeting the classical and newly described staphylococcal enterotoxin genes.

	Primers set and sequences (5′-3′)	Gene	Amplicon (bp)	References
SEA	TTGGAAACGGTTAAAACGAA	sea	120	[17]
SEA	GAACCTTCCCATCAAAAACA
SEB	TCGCATCAAACTGACAAACG	seb	478	[17]
SEB	GCAGGTACTCTATAAGTGCC
SEC	GACATAAAAGCTAGGAATTT	sec	257	[17]
SEC	AAATCGGATTAACATTATCC
SED	CTAGTTTGGTAATATCTCCT	sed	317	[17]
SED	TAATGCTATATCTTATAGGG
SEE	TAGATAAAGTTAAAACAAGC	see	170	[17]
SEE	TAACTTACCGTGGACCCTTC
SEG	TGCTATCGACACACTACAACC	seg	704	[18]
SEG	CCAGATTCAAATGCAGAACC
SEH	CGAAAGCAGAAGATTTACACG	seh	495	[18]
SEH	GACCTTTACTTATTTCGCTGTC
SEI	GACAACAAAACTGTCGAAACTG	sei	630	[18]
SEI	CCATATTCTTTGCCTTTACCAG
SElJ	CAGCGATAGCAAAAATGAAACA	selj	426	[19]
SElJ	TCTAGCGGAACAACAGTTCTGA
SElM	CCAATTGAAGACCACCAAAG	selm	517	[20]
SElM	CTTGTCCTGTTCCAGTATCA
SElN	ATTGTTCTACATAGCTGCAA	seln	682	[20]
SElN	TTGAAAAAACTCTGCTCCCA
SElO	AGTCAAGTGTAGACCCTATT	selo	534	[20]
SElO	TATGCTCCGAATGAGAATGA
SElK	ATGAATCTTATGATTTAATTTCAGAATCAA	selk	545	[21]
SElK	ATTTATATCGTTTCTTTATAAGAAATATCG
SElQ	GGAAAATACACTTTATATTCACAGTTTCA	selq	539	[21]
SElQ	ATTTATTCAGTTTTCTCATATGAAATCTC
SElR	AATGGCTCTAAAATTGATGG	selr	363	[22]
SElR	TCTTGTACCGTAACCGTTTT
SElU	AATGGCTCTAAAATTGATGG	selu	215	[22]
SElU	ATTTGATTTCCATCATGCTC
TSST-1	ATGGCAGCATCAGCTTGATA	tstH1	350	[17]
TSST-1	TTTCCAATAACCACCCGTTT
16S rDNA	ATA AGA CTG GGA TAA CTT CGG G	16SrDNA	500	[23]
16S rDNA	CTT TGA GTT TCA ACC TTG CGG TCG

f: forward; r: reverse.