Research Article
Safety Evaluation of the Coagulase-Negative Staphylococci Microbiota of Salami: Superantigenic Toxin Production and Antimicrobial Resistance
Table 1
Primer set for V3 16S rDNA sequencing and PCR/real time RT-PCR tests targeting the classical and newly described staphylococcal enterotoxin genes.
| | Primers set and sequences (5′-3′) | Gene | Amplicon (bp) | References |
| SEA | TTGGAAACGGTTAAAACGAA | sea | 120 |
[17] | SEA | GAACCTTCCCATCAAAAACA |
| SEB | TCGCATCAAACTGACAAACG | seb | 478 |
[17] | SEB | GCAGGTACTCTATAAGTGCC |
| SEC | GACATAAAAGCTAGGAATTT | sec | 257 |
[17] | SEC | AAATCGGATTAACATTATCC |
| SED | CTAGTTTGGTAATATCTCCT | sed | 317 |
[17] | SED | TAATGCTATATCTTATAGGG |
| SEE | TAGATAAAGTTAAAACAAGC | see | 170 |
[17] | SEE | TAACTTACCGTGGACCCTTC |
| SEG | TGCTATCGACACACTACAACC | seg | 704 |
[18] | SEG | CCAGATTCAAATGCAGAACC |
| SEH | CGAAAGCAGAAGATTTACACG | seh | 495 |
[18] | SEH | GACCTTTACTTATTTCGCTGTC |
| SEI | GACAACAAAACTGTCGAAACTG | sei | 630 |
[18] | SEI | CCATATTCTTTGCCTTTACCAG |
| SElJ | CAGCGATAGCAAAAATGAAACA | selj | 426 |
[19] | SElJ | TCTAGCGGAACAACAGTTCTGA |
| SElM | CCAATTGAAGACCACCAAAG | selm | 517 |
[20] | SElM | CTTGTCCTGTTCCAGTATCA |
| SElN | ATTGTTCTACATAGCTGCAA | seln | 682 |
[20] | SElN | TTGAAAAAACTCTGCTCCCA |
| SElO | AGTCAAGTGTAGACCCTATT | selo | 534 |
[20] | SElO | TATGCTCCGAATGAGAATGA |
| SElK | ATGAATCTTATGATTTAATTTCAGAATCAA | selk | 545 |
[21] | SElK | ATTTATATCGTTTCTTTATAAGAAATATCG |
| SElQ | GGAAAATACACTTTATATTCACAGTTTCA | selq | 539 |
[21] | SElQ | ATTTATTCAGTTTTCTCATATGAAATCTC |
| SElR | AATGGCTCTAAAATTGATGG | selr | 363 |
[22] | SElR | TCTTGTACCGTAACCGTTTT |
| SElU | AATGGCTCTAAAATTGATGG | selu | 215 |
[22] | SElU | ATTTGATTTCCATCATGCTC |
| TSST-1 | ATGGCAGCATCAGCTTGATA | tstH1 | 350 |
[17] | TSST-1 | TTTCCAATAACCACCCGTTT |
| 16S rDNA | ATA AGA CTG GGA TAA CTT CGG G | 16SrDNA | 500 |
[23] | 16S rDNA | CTT TGA GTT TCA ACC TTG CGG TCG |
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f: forward; r: reverse.
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