BioMed Research International / 2015 / Article / Fig 5

Research Article

{2-[1-(3-Methoxycarbonylmethyl-1H-indol-2-yl)-1-methyl-ethyl]-1H-indol-3-yl}-acetic Acid Methyl Ester Inhibited Hepatocellular Carcinoma Growth in Bel-7402 Cells and Its Resistant Variants by Activation of NOX4 and SIRT3

Figure 5

The upregulation of SIRT3 in HCC cells exposed to MIAM. (a) HCC cells were exposed to increasing concentration of MIAM for 72 h. Western blotting analyzed the expression of SIRT3 and p53 in HCC cells. Data are representative of 3 independent experiments. (b) The silencing of SIRT3 by transfection with siRNA of SIRT3 in Bel-7402/5FU cells (Figure 5(b), left). Bel-7402/5FU cells were incubated with MIAM for 48 h (Figure 5(b), right). Viable cells were estimated by the MTT assay and denoted as a percentage of untreated controls at the concurrent time point. The bars indicate mean ± S.D. (). (c) Overexpression of SIRT3 by transfection with human SIRT3 adenovirus (Ad-SIRT3) or control adenovirus expressing GFP (Ad-GFP) in Bel-7402/5FU cells (Figure 5(c), left); and then cells were incubated with MIAM for 48 h (Figure 5(c), right). Viable cells were estimated by the MTT assay and denoted as a percentage of untreated controls at the concurrent time point. , versus the vehicle control. The bars indicate mean ± S.D. ().
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