Disturbance of Oligodendrocyte Function Plays a Key Role in the Pathogenesis of Schizophrenia and Major Depressive Disorder

Figure 2

DISC1 overexpression inhibits oligodendrocyte differentiation. (a) Primary cultured rat OPCs infected with GFP-Adv or DISC1-GFP-Adv were lysed at 0, 24, 48, 72, 96, and 120 hours after PDGF deprivation and subjected to western blot analysis. (b) Primary cultured rat OPCs were immunostained with anti-GFP and anti-CNPase antibodies. The percentage of CNPase positive cells relative to the total number of GFP positive cells is shown. Infected cells from three experiments were analyzed. versus GFP-Adv. Scale bar = 100 μm. (c) Primary cultured rat OPCs were immunostained with anti-GFP and anti-β-tubulin antibodies for morphological observation. Infected cells from three independent cultures were classified according to their morphology (simple, intermediate, or complex) and quantified. The percentage of cells within each category, relative to the total number of GFP positive cells, is shown. versus GFP-Adv. Scale bar = 50 μm. (a)–(c) are adapted with permission from Hattori et al. [20].

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