miR-126 Is Involved in Vascular Remodeling under Laminar Shear Stress

Figure 2

LSS induces SDC-1, SDC-4, and VCAM-1 expression in HUVECs. (a) Representative immunofluorescence staining of SDC-1, SDC-4, and VCAM-1 protein on endothelial cells cultured under static or LSS conditions for 10 min and 24 h. HUVEC cells were immunolabeled with anti-SDC-1 (A1–3), anti-SDC-4 (B1–3), and anti-VCAM-1 (C1–3) antibodies (green) or with their respective control isotypes. The nuclei were stained with DAPI (blue). High resolution zoom images of cells were added to each figure. Scale bar = 20 μm. (b) Immunofluorescence quantification of proteins normalized with the nuclei number () was done and 5 different fields in HUVECs submitted to static conditions and LSS (10 min and 24 h) were counted and presented as fluorescence intensity/nuclei (AU: arbitrary units). A progressive increase of SDC-1 and SDC-4 expression up to 24 h and an increase at 10 min and then a decrease of VCAM-1 expression were observed. One-way Global ANOVA and Student’s -test, for SDC-1: (10 min of LSS versus static), (24 h of LSS versus static); for SDC-4: (10 min of LSS versus static), (24 h of LSS versus static); for VCAM-1: (10 min of LSS versus static), (24 h of LSS versus static).

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