Sialic Acid Expression in the Mosquito Aedes aegypti and Its Possible Role in Dengue Virus-Vector Interactions

Figure 5

DENV overlay assay with Ae. aegypti SG protein extract (SGPE) and Aedes saliva in the presence or absence of C. perfringens sialidase. (a) DENV-SGPE interactions. Lanes 1–6 show nitrocellulose membranes stained with Ponceau red. Lane 1: MW markers; lane 2: SGPE; lane 3: SGPE pretreated with sialidase; lane 4: fetuin glycoprotein; lane 5: asialofetuin; and lane 6: fetuin pretreated with sialidase. Lanes 7–12 show the blot and overlay assays of SGPE. Lane 7: MW markers; lane 8: SGPE; lane 9: blot of SGPE with SNA lectin; lane 10: DENV overlay with SGPE; lane 11: DENV overlay with SGPE pretreated with sialidase; lane 12: blot of fetuin glycoprotein with SNA lectin; lane 13: blot of asialofetuin with SNA lectin; and lane 14: blot of SNA lectin with fetuin pretreated with sialidase. (b) DENV-saliva interactions. Lane 1: blot of mosquito saliva with SNA lectin; lane 2: DENV overlay with saliva pretreated with sialidase; and lane 3: overlay of DENV-saliva proteins.

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