Abstract
Hsp70s (heat shock protein 70s) are a class of molecular chaperones that are highly conserved and ubiquitous in organisms ranging from microorganisms to plants and humans. Most research on Hsp70s has focused on the mechanisms of their functions as molecular chaperones, but recently, studies on stress responses are coming to the forefront. Hsp70s play key roles in cellular development and protecting living organisms from environmental stresses such as heat, drought, salinity, acidity, and cold. Moreover, functions of human Hsp70s are related to diseases including neurological disorders, cancer, and virus infection. In this review, we provide an overview of the specific roles of Hsp70s in response to stress, particularly abiotic stress, in all living organisms.
1. Introduction
Proteins fold into the correct three-dimensional structures with the assistance of chaperone proteins to avoid abnormal folding and aggregation [1]. Heat shock proteins (Hsps), molecular chaperones originally discovered based on their upregulation in response to heat stress, have been divided into five different families based on molecular weight: Hsp100, Hsp90, Hsp70, Hsp60, and small (s) Hsp [2]. Living organisms must adapt to temperature changes during their lifetime, and the Hsp70s are found in every kingdom. For instance, photosynthetic eukaryotes contain four types of Hsp70s which localize to different cellular compartments: cytoplasm, mitochondrion (MT), chloroplast (CP), and endoplasmic reticulum (ER) [3]. Hsp70s are involved in many cellular processes, including protein folding, protein translocation across membranes, and regulation of protein degradation. This review will focus on types and numbers of Hsp70s in various kingdoms species, as well as their functions in stress responses.
2. Structure and Isotype of Hsp70s
Hsps are pivotal molecular chaperones that function in response to proteotoxic stress by refolding misfolded proteins. Hsp70 (called DnaK in prokaryotes) is a 70-kDa protein that represents the best-known heat shock protein [4]. Hsp70s are highly conserved and exist in all organisms ranging from microorganisms to plants and humans. Structurally, Hsp70s contain a nucleotide-binding domain (NBD), acting as a ATPase domain, a substrate-binding domain (SBD), and a C-terminal lid region that covers the SBD. Usually, the NBD is around 45 kDa and the SBD is around 15 kDa. By contrast, the length or sequence of the C-terminal region varies and in some cases contains organelle-specific retention motifs [5]. The NBD and SBD are highly conserved, while the C-terminal region has varied to some extent (Figure 1).
In Escherichia coli, there are three Hsp70 proteins: DnaK, HscA (Hsc66), and HscC (Hsc62) [5]. Of 14 Hsp70 proteins in Saccharomyces cerevisiae, 9 are present in the cytosol, 3 are found in mitochondria (Ssc, Ecm, and Ssq), and 2 localize to the ER (Kar2 and Lhs1) [6]. In the unicellular alga Chlamydomonas reinhardtii, a total of 7 genes encode Hsp70s, with Hsp70A and Hsp70E as cytosolic proteins, Hsp70B in the chloroplast, Hsp70C and Hsp70D localize to mitochondria, and BiP1 and BiP2 are found in the ER lumen [7]. Chlamydomonas is related to the last common ancestor of plants and animals; in contrast to the land plants that have numerous cytoplasmic Hsp70s, the alga has only one cytoplasmic Hsp70. Therefore, it seems that evolutionary and environmental selection pressure drove an increase in the types and numbers of molecular chaperones [8].
Nine cytosolic Hsp70 family members are encoded in the genome of the moss Physcomitrella patens, which is evolutionarily placed among the first group of plants to transition from an aquatic to a terrestrial lifestyle [9]. In addition, three chloroplast and four mitochondrial Hsp70 genes have been identified in moss, and knockout of cpHsp70-2 was found to be lethal [10]. The expansion of the Hsp70 gene family was reported to result from genome and gene duplications, which are a driving force in eukaryotic evolution [11]. Rice (Oryza sativa) contains 32 Hsp70 genes; among these are 24 Hsp70 family and 8 Hsp110 subfamily members [12]. The Arabidopsis Hsp70 gene family includes 18 members, 14 in the DnaK subfamily and 4 in the Hsp110/SSE subfamily. Of the DnaK subfamily proteins, six are in the cytosol, three are in chloroplast, three are in the ER, and two are in mitochondria [13]. Similarly, the Populus trichocarpa genome includes 20 Hsp70 genes [14]. As demonstrated by the Hsp70 family, it appears that the complexity and function of plant genomes probably evolved after land colonization.
Studies of the Hsp70 gene family in animals have also revealed interesting findings. In Drosophila melanogaster, the Hsp70 family includes seven genes in the cytoplasmic group, but only one ER gene and one mitochondrial gene [15]. Besides that, at least 13 Hsp70 homologs are present in human, including Hsp70-5 (known as Grp78 or BiP), Hsp70-9 (known as mortalin, GRP75, or mtHsp70), and other Hsp70s in the cytosol or nucleus [16]. Based on data from fish [17], chicken [18], and buffalo [19], it appears that vertebrate animals generally have only one ER gene and that the stress-induced Hsp70s are mainly cytosolic members.
3. Hsp70s in Responses to Biotic and Abiotic Stress
Increasing evidences show that Hsp70s are involved in folding newly synthesized proteins, signal transduction, and protein translocation from nucleus to organelles. As housekeeping genes, Hsp70s are responsible for essential functions in response to various biotic and abiotic stresses.
3.1. Hsp70s in Prokaryotes
Hsp70, generally called DnaK in bacteria, is important for responses to several abiotic stresses. The expression of DnaK increases under heat, acidity, and cold stresses in Alicyclobacillus acidoterrestris, which is a Gram positive, rod-shaped, aerobic, acidophilic, thermophilic, and spore-forming bacterium [20]. Studies in Staphylococcus aureus indicated that the DnaK system contributes to heat and oxidative stress tolerance, and DnaK protein refolding machinery plays an essential role in the stress responses [21]. Hsp70/DnaK bind pathogen-associated molecular patterns (PAMPs), such as endotoxin lipopolysaccharide (LPS) and potent antimicrobial substances, which suppress pathogen proliferation [22]. Since DnaK carries out key functions to enhance shrimp resistance against the pathogen Vibrio harveyi, which infects aquatic animals, bacteria overproducing DnaK have been added into feed pellets to protect the white leg shrimp Penaeus vannamei in aquaculture [23].
3.2. Hsp70s in Fungi and Plants
Hsp70s were first discovered in fruit flies in the 1960s when a lab worker accidentally increased the incubation temperature, and the basic function of these proteins is recognized to be as molecular chaperones [24]. Hsp70s are evolutionarily highly conserved. In plants, mitochondrial and plastid Hsp70s have relatively high sequence similarity to DnaK (the bacterial Hsp70 homolog), ranging from 50% to 63%, while the other Hsp70s members are more closely related to cytosolic Hsp70s [25]. The cytosolic Hsp70 family members display up to 75% amino acid identity to other plant Hsp70 proteins of this group [26]. The sequences of Hsp70 homologs from the different compartmentations have similarity and peculiarity with each other. According to those foregoing researches, ER arise from the early stages of the eukaryotic cell, while mitochondria and chloroplast are the accepted endosymbiotic origin organelles. Here we showed an example in rice (Figure 2). From the phylogenetic tree of Hsp70 family in rice, we can clearly see that the evolutionary relation of those Hsp70s is consistent with the origin of compartmentations.
3.2.1. Roles of Cytosolic Hsp70s
The cytosolic Hsp70 proteins can be divided into two subgroups. One group is the heat shock cognate (Hsc) 70 proteins, which are involved in normal cellular activities such as protein folding and intracellular targeting. Another group is Hsp70s, which function in responses to various stresses and probably participate in the refolding of denatured or aggregated proteins [6].
Saccharomyces cerevisiae contains four classes of cytosolic Hsp70, termed Ssa, Ssb, Sse, and Ssz. The four members of Ssa in S. cerevisiae represent the major cytosolic Hsp70 family, and among them Ssa3 and Ssa4 confer greater resistance to H2O2 [27]. The Hsp70 Ssz1 can bind the Hsp40 Zuotin and form a heterodimeric ribosome-associated complex (known as RAC) to stimulate Ssb ATPase activity [28]. Moreover, Ssb might affect membrane protein biogenesis in response to salt [29]. Sse1 and Sse2 proteins represent the Hsp110 subfamily in S. cerevisiae. Loss of Sse1 renders cells slow growing and temperature sensitive, and Sse1 and Sse2 constitute an essential gene pair, as inactivation of both genes is lethal [30].
As a single-celled chlorophyte green alga, Chlamydomonas lives in diverse environmental conditions around the world. It has been demonstrated that Chlamydomonas Hsp70 genes are induced by light, and the expression regulation of cytosolic Hsp70A is mediated by a heat shock-independent pathway [31]. In maize, cytosolic Hsp70 regulates the abscisic acid- (ABA-) induced antioxidant response during drought and heat stress. Upon exposure to abiotic stresses, H2O2 production is induced and leads to enhanced activities of antioxidant enzymes, with subsequent increases in the endogenous ABA levels and Hsp70 accumulation [32].
To identify the functions of Hsp110/SSE subfamily members in Arabidopsis, artificial microRNA (amiRNA) technology has been used to silence AtHsp70-14 and AtHsp70-15 genes. The transgenic amiRNA-AtHsp70-14/15 plants showed growth retardation and their leaves shriveled faster than those of the control. Besides, AtHsp70-15-deficient plants were more sensitive to ABA and heat treatment [33].
In short, the cytosolic Hsp70s in plants and fungi serve as key factors in responses to ROS, salt, and ABA stresses.
3.2.2. Roles of ER Hsp70s
The accumulation of unfolded or misfolded proteins in the ER lumen is thought to cause ER stress and activate a protective signaling pathway known as the unfolded protein response (UPR) [34], which involves ER-resident molecular chaperone Hsp70s called Binding immunoglobulin Proteins (BiPs). The unfolded or misfolded proteins are recognized and sent out of the organelle for degradation.
Overexpression of BiP in transgenic tobacco (Nicotiana tabacum L. cv Havana) plants can prevent cellular dehydration, reducing the effects of drought [35]. Nevertheless, soybean BiP functions in drought tolerance and drought-induced leaf senescence [36]. Compared to wild type, the rates of photosynthesis and CO2 assimilation are less inhibited by drought in transgenic lines overexpressing BiP, and these lines are resistant to drought, although the mechanism is unclear [36].
In Citrus, the ER-resident Hsp70-5 shows seed-specific expression and has a key function in seed desiccation tolerance. Furthermore, Citrus Hsp70-5 is highly expressed in response to biotic stresses, such as Xylella infection and Citrus tristeza viral infection, due to activation of the UPR signaling pathway [37].
The mechanism of BiP-mediated stress resistance is not clear at all; therefore, the general mechanisms for protection of cellular components during plant cell stresses require further investigation.
3.2.3. Roles of Mitochondrial Hsp70s
Most mitochondrial protein precursors are synthesized in the cytosol and subsequently imported into the mitochondrial matrix. It has been demonstrated that mtHsp70 plays a prominent role in mitochondrial biogenesis. According to previous reports, mtHsp70 binds to protein precursors as they come into the matrix and provides the driving force for the import reaction through ATP hydrolysis. After import, mtHsp70 probably participates in substrate refolding [10]. In pea (Pisum sativum) leaves, mtHsp70 function has been demonstrated to be related to cellular development, and the expression of mtHsp70 is suppressed as the plant ages [38].
Studies of mtHsp70 functions in response to abiotic stress in plants are limited, although mtHsp70 genes have been identified from many plant species. The moss Pohlia nutans lives in the Antarctic polar environment, and PnHsp70, encoding a mitochondrial chaperone, is induced by low or high temperature, salinity, drought, or PEG treatment, as well as UV radiation [39].
In general, although the functions of plant mitochondrial Hsp70 proteins under stress conditions have not been well studied, they are known to be crucial for cell survival.
3.2.4. Roles of Chloroplast Hsp70s
In Chlamydomonas, the chloroplast-localized Hsp70B plays a key role in protection against photoinhibition. The expression of Hsp70B is increased not only under heat shock, but also under high light or oxidative stress conditions [40]. Hsp70s are important in pathological processes at the same time. A wheat (Triticum aestivum L.) chloroplast TaHsp70 plays a crucial role in stress-related responses and in defense responses elicited by infection with stripe rust fungus via a JA-dependent signal transduction pathway [41]. During Arabidopsis seed germination, cpHsp70-1 is important for root growth in response to the heat treatment. Analyses of cpHsp70-1 and cpHsp70-2 single knockout mutants indicated that cpHsp70s are essential for plant development and that the two cpHsp70s most likely have redundant but also distinct functions [42], while cpHsp70-1 is more highly expressed in leaf than all other Hsp70s, suggesting a specific role of cpHsc70-1 in chloroplasts [43]. Similarly in rice, OsHspCP1 plays an important role in chloroplast development during heat stress [44].
In summary, the chloroplast Hsp70s carry out pivotal functions in processes related to growth and development and are regulated by environmental factors such as light, heat, and pathological stresses.
3.3. Hsp70s in Animals
Hsp70 represents the best-characterized class of cytoprotection proteins, and its expression is markedly induced by a variety of stresses, such as heat shock, UV irradiation, and exposure to heavy metals. However, the distribution and accumulation of Hsp70s are highly tissue specific in different development stages of animals.
In insects (e.g., Drosophila melanogaster), zebrafish (Danio rerio), and mammals, Hsp70 has been shown to be expressed in a stage- and tissue-specific manner. In spermatogonial cells of D. melanogaster, the constitutive presence of Hsp70 proteins was shown to be more stable than in other cell types during heat shock [45]. In bull spermatozoa, there is a positive correlation between Hsp70 expression level and sperm motility [46]. Interestingly, in zebrafish embryos, in situ hybridization experiments indicated that the amount of Hsp70 mRNA localized in the brain, eye, otic vesicle, and yolk sac was enhanced under heat stress conditions [47]. In rainbow trout, Hsp70 accumulates in juvenile trout tissues including the gill and liver upon exposure to heavy metals [48].
In pigs, the expression level of Hsp70 in blood lymphocytes increased significantly under cold stress at −10 to −4°C, and it can serve as a molecular biomarker of cold stress in animals [49].
Among human Hsp70 family members, the ER-located Hsp70-5 and cytosolic Hsp70-8 are regarded as essential housekeeping genes [50]. Furthermore, the mitochondrial Hsp70-9 participates in several cellular processes related to maintaining protein homeostasis. In mice, overexpression of mtHsp70 in retinal ganglion cells (RGCs) can suppress vision loss and optic atrophy. In the near future, mtHsp70 will likely emerge as a therapeutic target for optic neuritis, Parkinson’s and Alzheimer’s diseases, and cancers [51], whereas human Hsp70-1 and Hsp70-2 also display different functions in different cancer cells. Hsp70-1 is related to the origins of malignant tumors and may be a relevant cancer survival protein. By contrast, Hsp70-2 is abundantly expressed in the nervous system, and its expression is increased in metastatic breast cancers [52].
The human Hsp70s have attracted extensive attention worldwide, because they present a new approach for treatment of various kinds of diseases affecting mitochondria.
4. Conclusions
Hsp70s are widely distributed in numerous organisms from bacteria to animals. They play important roles in essential cellular processes and also in stress-related processes of the cells. In this review, we summarized their functions and application in response to a variety of stresses in bacteria, fungi, plants, and animals. Given that Hsp70 is also a potential therapeutic target for a variety of human diseases, further studies will be required to fully elucidate the roles of Hsp70s in all living species.
Conflict of Interests
The authors declare that they have no competing interests regarding the publication of this paper.
Acknowledgment
This work was supported by 100 Talents Program of The Chinese Academy of Sciences and the National Natural Science Foundation of China (31571262).
Supplementary Materials
The Supplementary Data summarizes the information of all Hsp70s mentioned in the text, including the gene names, genes locus, cellular localization and so on.