Integrative Analysis of CRISPR/Cas9 Target Sites in the Human HBB Gene

Figure 6

T7E1 mutation detection assays for the cleavage activity of the Cas9/I2-1 system in 293T cells. (a) On-target cleavage activities of the Cas9/I2-1 system by different concentrations. The mutated rate is quantified by ImageJ. (b) Off-target cleavage activities of the Cas9/I2-1 system ().

(a)

(b)