The Use of an IL-1 Receptor Antagonist Peptide to Control Inflammation in the Treatment of Corneal Limbal Epithelial Stem Cell Deficiency

Figure 7

IL-1β gene expression in LPS stimulated keratocytes treated with peptide was compared to LPS stimulated, nonpeptide treated, and non-LPS stimulated controls using q-PCR. Relative genetic expression of IL-1β was compared to expression of the GAPDH housekeeping gene. Keratocytes stimulated with LPS demonstrated a significant increase in IL-1β gene expression compared to the non-LPS stimulated control. The addition of 1000 μg/mL peptide significantly reduced IL-1β expression to that of the non-LPS stimulated control (, mean ± sd).