MALDI-TOF MS and CD Spectral Analysis for Identification and Structure Prediction of a Purified, Novel, Organic Solvent Stable, Fibrinolytic Metalloprotease from Bacillus cereus B80

Table 1

Purification steps for metalloprotease.

	Activity (U)	Protein (mg)	Sp. activity U/mg	Recovery	Fold purification
Crude enzyme	31773.5	117.12	271.29	100	1
EtOH treated	31773.1	87.2	364.76	100	1.34
Sephadex	15682.7	10.4	1507.95	49.35	5.55
Q Sepharose	10668.9	3	3556.3	33.577	13.10