Effect of Fe₃O₄ Nanoparticles on Skin Tumor Cells and Dermal Fibroblasts

Figure 4

(a) Reactive oxygen species and carbonylated proteins. Tumor cells were untreated or incubated with 350 μM Fe₃O₄ nanoparticles for 24 h. Carbonylated proteins were evaluated by western blot analysis. 250 μM H₂O₂ was incubated for 1 h and served as positive control and α-tubulin served as loading control. . CM: conditioned medium. versus untreated control (ANOVA, Dunnett’s test). (b) Detection of 8-isoprostane in SCL-1 cell supernatants. Subconfluent SCL-1 cells were incubated with Fe₃O₄ nanoparticles (50 μM, 350 μM) for 4 h and 12 h and with 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH, 10 mM, radical initiator) for 4 h as a positive control. The cell culture supernatants were collected and a competitive ELISA assay applied. The 8-isoprostane concentration of untreated control was set at 1. The data represent the mean ± s.e.m. . versus untreated control () (Student’s -test). (c) MDA formation in SCL-1. MDA formation as a marker for lipid peroxidation was determined by HPLC. Subconfluent SCL-1 cells were treated with Fe₃O₄ nanoparticles (50 μM, 350 μM) for 4 h and 12 h and with AAPH (10 mM, radical initiator) for 4 h as a positive control. The data represent the fold increase over control, which was set at 1. The data represent the mean ± s.e.m. . versus untreated control (ANOVA, Dunnett’s test).

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