Inactivation of Src-to-Ezrin Pathway: A Possible Mechanism in the Ouabain-Mediated Inhibition of A549 Cell Migration

Figure 4

Effect of ouabain on migration of A549 cells. (a) In vitro migration assay was performed twice in triplicate using a 48-well Boyden chamber with a gelatin-coated polycarbonate membrane. DMEM containing either 0.1% FBS or 10% FBS was added into the bottom chamber and cells were loaded into the upper chamber and incubated at 37°C for 6 h. The cells on the upper side of the membrane were removed, and the cells on the bottom of the filter membrane were stained with Diff-Quick solution. (b) The numbers of migrated cells were counted under a light microscope. The data are presented as mean ± standard deviation (; ; ).

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