Regulation of ICAM-1 in Cells of the Monocyte/Macrophage System in Microgravity

Figure 6

ICAM-1 expression in macrophage-like differentiated U937 cells after long-term exposure to microgravity during the SIMBOX/Shenzhou-8 mission. Cells were cultured under standard cell culture conditions (incubator control) or exposed to different gravity conditions during the SIMBOX/Shenzhou-8 mission. Differentiated U937 cells were fixed in microgravity (μg group) or in 1 g (1 g control group) after 5 days. Only CellMask-positive and TUNEL-negative cells were analyzed. (a) Each group represents analysis of the mean fluorescence of 200–1000 individual cells from one recovered slide. Data are expressed as the median of mean single cell fluorescence intensities with the smallest observation (sample minimum), lower quartile, median, upper quartile, and largest observation (sample maximum). Statistical analysis was performed with GraphPad Prism 5, Wilcoxon test, , , and . (b) Standard cell culture control (con), 1 g hardware control (1 g) and the microgravity sample (μg).

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