Regulation of ICAM-1 in Cells of the Monocyte/Macrophage System in Microgravity

Table 1

Regulation of ICAM-1 in cells of the monocyte/macrophage system in microgravity. Overview about cell types, microgravity platforms, the experiment durations, analysis methods, number of experiments, and effects on ICAM-1.

Experimental platform	Cell types	Experimental groups	Analysis method	Number of replicates	Time of μg exposal	Regulation of ICAM-1
						μg/1 g	1 g/incubator control
Clinorotation	BV2; microglia cells	μg group; 1 g control group	FACS; (20.000 events/sample)	= 3	24 h
	Primary; macrophages	μg group; 1 g control group	FACS; (5.000 events/sample)	= 3	1–5 d
	U937; macrophages	μg group; 1 g control group	FACS; (10.000 events/sample)	= 6	1–5 d
Parabolic flight	BV2; microglia cells	μg group; 1 g control group	Confocal microscopy/; random sampling module; (>500 cells/sample)	= 3 (w/o)
				= 3 (with PMA)	20 sec
				= 3 (with TNF-a)		—
	U937; macrophages	μg group; 1 g control group; Ground control	FACS; (10.000 events/sample)	= 9	20 sec
				= 9
				= 6
	U937; monocytes	μg group; 1 g control group; Ground control	FACS; (10.000 events/sample)	= 9 (w/o PMA)	20 sec	—	—
				= 5 (with PMA)
				= 12 (w/o PMA)
				= 6 (with PMA)
				= 2 (w/o PMA)
				= 1 (with PMA)
		μg group; 1 g control group; 1.8 g control group; Hardware control	Microarray	= 8	20 sec	—	—
				= 8
				= 6
				= 6
	Primary; macrophages	μg group; 1 g control group	FACS; (10.000 events/sample)	= 9	20 sec	—
				= 9
SIMBOX; Shenzhou 8	U937; Macrophages	μg group; 1 g control group; Incubator control	Confocal microscopy; (300 cells/sample); (650 cells/sample); (1000 cells/sample)	= 1	5 d
TEXUS-49	U937; monocytes	μg group; baseline group; Hardware control	Microarray	= 7	10 min	—	—
				= 5
				= 6