Acoustic-Frequency Vibratory Stimulation Regulates the Balance between Osteogenesis and Adipogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells

Figure 3

Acoustic-frequency vibratory stimulation (AFVS) modulates expression of mRNA encoding osteogenesis-specific markers in human bone marrow-derived mesenchymal stem cells (BM-MSCs) at the time points of day 7 ((a), (b), (c), and (d)) and day 14 ((e), (f), (g), and (h)). The mRNA levels of COL1A1 ((a), (e)), ALP ((b), (f)), RUNX2 ((c), (g)), and SPP1 ((d), (h)) were measured by real-time RT-PCR. Values are mean ± standard error of four independent experiments (). , in the indicated groups from unpaired -test. , compared with the 0 Hz control group from unpaired -test.

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