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BioMed Research International
Volume 2015, Article ID 543163, 7 pages
http://dx.doi.org/10.1155/2015/543163
Research Article

The Correlation between miRNA and Lymph Node Metastasis in Gastric Cancer

1Division of General Surgery, Department of Surgery, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Road, Beitou District, Taipei City 11217, Taiwan
2School of Medicine, National Yang-Ming University, Taipei City 11221, Taiwan
3Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei City 11221, Taiwan
4Division of Colon & Rectal Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei City 11217, Taiwan
5En Chu Kong Hospital, New Taipei City 237, Taiwan
6National Yang-Ming University Hospital, Yilan City 260, Taiwan
7Department of Pathology, Taipei Veterans General Hospital, Taipei City 11217, Taiwan
8Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei City 11217, Taiwan
9Institute of Pharmacology, National Yang-Ming University, Taipei City 11221, Taiwan

Received 26 June 2014; Revised 12 October 2014; Accepted 15 October 2014

Academic Editor: Sachiyo Nomura

Copyright © 2015 Kuo-Hung Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Lymph node metastasis (LNM) in gastric cancer is associated with higher rate of cancer recurrence and poor prognosis. As a result, a reliable biomarker for the prediction of LNM is important and would be valuable in the clinical practice. MiRNA microarray revealed that ten miRNAs were expressed significantly different among patients with or without LNM. A total of 46 gastric cancer patients were enrolled and divided into two groups (23 in each group) according to the presence or absence of LNM. RT-PCR of these 10 miRNAs was investigated and compared between the two groups. MiR-1207-5p was significantly upregulated in gastric cancer patients without LNM compared with those with LNM. Patients with upregulated miR-1207-5p had less scirrhous stromal reaction, less lymphovascular invasion, and earlier pathological T category, N category, and TNM stage, compared with those with downregulated or unchanged miR-1207-5p. Multivariate analysis showed that stromal reaction type, lymphovascular invasion, pathological T category and TNM stage, and expression of miR-1207-5p were independent risk factors of LNM. MiR-1207-5p could serve as a useful biomarker in the prediction of LNM in gastric cancer.