Occurrence of Multidrug Resistant Extended Spectrum Beta-Lactamase-Producing Bacteria on Iceberg Lettuce Retailed for Human Consumption

Figure 4

Gel electrophoresis and Southern hybridization of donors and transconjugants. (a) DNA isolated from an environmental strain of K. pneumoniae and reference strains of E. coli and K. pneumoniae and (b) Southern hybridization of an environmental strain of K. pneumoniae using the probe. Lane R: reagent control, Lane M: E. coli V517 plasmids, Lane 1: K. pneumoniae ATCC 700603 plasmids (used as a reference), Lane 2: K. pneumoniae T1 plasmids, and Lane D: DIG-labeled DNA ladder III (Roche), a linear DNA.

(a)

(b)