Occurrence of Multidrug Resistant Extended Spectrum Beta-Lactamase-Producing Bacteria on Iceberg Lettuce Retailed for Human Consumption

Table 1

Primer sequences used in PCR amplification of ESBL and 16S rRNA gene sequences.

Target gene	Primer sequences (5′-3′)	(°C)	Expected amplicon size (bp)	Reference
16S rRNA	F: AGAGTTTGATCMTGGCTCAG	60	1400	[38]
	R: AAGGAGGTGATCCAGCC
blaSHV	F: GGTTATGCGTTATATTCGCC	60	867	[33]
	R: TTAGCGTTGCCAGTGCTC
blaTEM	F: CCGTGTCGCCCTTATTCC	56	800	[35]
	R: AGGCACCTATCTCAGCGA
blaCTX-M	F: TTTGCGATGTGCAGTACCAGTAA	56	500	[35]
	R: CTCCGCTGCCGGTTTTATC
blaCTX-M-1	F: AAAAATCACTGCGCCAGTTC	56	415	[35]
	R: AGCTTATTCATCGCCACGTT